Team:Goettingen/week21-2

From 2012.igem.org

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<b>V09_20_2 PCR of <i>fliC</i></b><br>
<b>V09_20_2 PCR of <i>fliC</i></b><br>
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<ul>
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<li>Experiment: <br>
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In order to get more <i>fliC</i>, the already quikchanged fliC was amplified via PCR. The PCR product was analyzed via gel-electrophoresis and subsequently purified using peqGOLD Gel Extraction Kit (Peqlab) according to the manual.<br>
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<b>V09_20_3 Repetition of preparative double digestion of <i>flhDC, FliC</i> and pSB1C3</b><br>
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<ul>
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<li>Experiment:<br> In order to clone the flhDC, FliC constructs into pSB1C3, all components were digested with EcoRI and PstI according to the protocol.<br>
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</ul>
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<b>V09_20_4 Preparation of over night cultures</b><br>
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<ul>
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<li>Experiment: <br>
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TeXT<br>
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</table>
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<table cellpadding="20 px" border="1" bordercolor="black" valign="top">
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<h2><b>V09_21 >/b></h2><br>
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<b> V09_21_1 Ligation and chemical Transformation of <i>flhDC, FliC</i> and <i> pSB1C3</i></b><br>
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<ul>
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<li>Experiment: <br>
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The restriction products were separated on a gel in order to verify the digestions success and purify the desired fragments. After ligation of the genes into pSB1C3, we transformed the ligated plasmids into <i>E. coli</i> DH10B according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a. <br>
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</ul>
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<b>V09_20_2 Mini preps</b><br>
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<ul>
<li>Experiment: <br>
<li>Experiment: <br>

Revision as of 09:03, 21 September 2012

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