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Team:Goettingen/week10-3
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<b>Startpoint of Saturated mutagenesis experiment!</b><br> | <b>Startpoint of Saturated mutagenesis experiment!</b><br> | ||
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| - | <li>Experiment: <br>We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances. A saturated mutagenesis PCR was performed to mutate five individual amino acid residues in the ligand binding site of TAR. The primers that are utilized are the following:<br> | + | <li>Experiment: <br>We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances. A saturated mutagenesis PCR was performed to mutate five individual amino acid residues in the ligand binding site of TAR. The primers that are utilized are the following:<br><br> |
TARR6973Fw <br>gcgcaGGAAAGGTCTCACTGAGTnnNTCAGCGGTAnnNATGATGATGGATTCCTCCAATCAACAAAG<br> | TARR6973Fw <br>gcgcaGGAAAGGTCTCACTGAGTnnNTCAGCGGTAnnNATGATGATGGATTCCTCCAATCAACAAAG<br> | ||
TARR6973Rv <br>gcgcaGAGTAGGTCTCATCAGGTTAATGCGCGTTTGCAG<br> | TARR6973Rv <br>gcgcaGAGTAGGTCTCATCAGGTTAATGCGCGTTTGCAG<br> | ||
Revision as of 09:15, 20 September 2012
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#3 Chemoreceptor Library - 10th WeekBack to overview
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