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Team:Goettingen/week10-3
From 2012.igem.org
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<li>Experiment: <br>We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances. A saturated mutagenesis PCR was performed to mutate five individual amino acid residues in the ligand binding site of TAR. The primers that are utilized are the following:<br> | <li>Experiment: <br>We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances. A saturated mutagenesis PCR was performed to mutate five individual amino acid residues in the ligand binding site of TAR. The primers that are utilized are the following:<br> | ||
| - | TARR6973Fw gcgcaGGAAAGGTCTCACTGAGTnnNTCAGCGGTAnnNATGATGATGGATTCCTCCAATCAACAAAG<br> | + | TARR6973Fw <br>gcgcaGGAAAGGTCTCACTGAGTnnNTCAGCGGTAnnNATGATGATGGATTCCTCCAATCAACAAAG<br> |
| - | TARR6973Rv gcgcaGAGTAGGTCTCATCAGGTTAATGCGCGTTTGCAG<br> | + | TARR6973Rv <br>gcgcaGAGTAGGTCTCATCAGGTTAATGCGCGTTTGCAG<br> |
| - | TARY149F150T154Fw gcgcaGGAAAGGTCTCAGGAGCTnnNnnNGCTCAGCCAnnNCAGGGAATGCAAAATGCAATGGGCGAAG<br> | + | TARY149F150T154Fw <br>gcgcaGGAAAGGTCTCAGGAGCTnnNnnNGCTCAGCCAnnNCAGGGAATGCAAAATGCAATGGGCGAAG<br> |
| - | TARY149F150T154Rv gcgcaGAGTAGGTCTCACTCCAGTATTGCCATAATCTAGGTAATC<br> | + | TARY149F150T154Rv <br>gcgcaGAGTAGGTCTCACTCCAGTATTGCCATAATCTAGGTAATC<br> |
</li> | </li> | ||
Revision as of 09:14, 20 September 2012
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