Team:Goettingen/week10-3

From 2012.igem.org

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<b>Startpoint of Saturated mutagenesis experiment!</b><br>
<b>Startpoint of Saturated mutagenesis experiment!</b><br>
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<ul>
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<li>Experiment: <br>We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances.   </li>
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<li>Experiment: <br>We aim to apply directed evolution to reprogram the bacterial chemoreceptor TAR in order to enable the perception of novel substances. A saturated mutagenesis PCR was performed to mutate five individual amino acid residues in the ligand binding site of TAR. The primers that are utilized are the following:<br>
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TARR6973Fw gcgcaGGAAAGGTCTCACTGAGTnnNTCAGCGGTAnnNATGATGATGGATTCCTCCAATCAACAAAG<br>
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TARR6973Rv gcgcaGAGTAGGTCTCATCAGGTTAATGCGCGTTTGCAG<br>
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TARY149F150T154Fw gcgcaGGAAAGGTCTCAGGAGCTnnNnnNGCTCAGCCAnnNCAGGGAATGCAAAATGCAATGGGCGAAG<br>
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TARY149F150T154Rv gcgcaGAGTAGGTCTCACTCCAGTATTGCCATAATCTAGGTAATC<br>
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</li>
</ul>
</ul>
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Revision as of 09:14, 20 September 2012

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