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Team:USTC-China/groupmeetings
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| - | <p>I. Discuss about our project: | + | <p>I. Discuss about our project:<br> |
1. SNS:<br> | 1. SNS:<br> | ||
| - | To complete this project is hardly possible. While it is likely to realize part of it, such as a system which is able to create and sense signals. But we need to confer our project some new meanings. | + | To complete this project is hardly possible. While it is likely to realize part of it, such as a system which is able to create and sense signals. But we need to confer our project some new meanings.<br> |
| - | 1) About making a signal disappear, according to Mr. Hong, we can add a rapid degradation label onto the signal. | + | 1) About making a signal disappear, according to Mr. Hong, we can add a rapid degradation label onto the signal.<br> |
| - | 2) We are afraid that the colony is too small to create an appropriate concentration gradient. According to Mr. Hong, it won’t be a big problem. | + | 2) We are afraid that the colony is too small to create an appropriate concentration gradient. According to Mr. Hong, it won’t be a big problem.<br> |
| - | + | <br> | |
| - | 2. Solve the graph colouring problem using E.coli | + | 2. Solve the graph colouring problem using E.coli<br> |
| - | 1) In fact, we will only use E.coli to emit light in different colors, and all the algorithm for solving graph coloring problem is processed artificially. | + | 1) In fact, we will only use E.coli to emit light in different colors, and all the algorithm for solving graph coloring problem is processed artificially.<br> |
| - | 2) We need to consult professors in Chemistry Department about constructing structures using ssDNA. | + | 2) We need to consult professors in Chemistry Department about constructing structures using ssDNA.<br> |
| - | 3) The scale of DNA strand is far too small than that of E.coli. | + | 3) The scale of DNA strand is far too small than that of E.coli. <br> |
| - | 4) The fusion protein of Zn finger and the Ag43 would be very useful. We can think about how to make use of it. | + | 4) The fusion protein of Zn finger and the Ag43 would be very useful. We can think about how to make use of it.<br> |
| - | + | <br> | |
| - | 3. Fatigue of specific stimulus | + | 3. Fatigue of specific stimulus<br> |
| - | 1) comK is a protein produced by bacillus subtilis and “competence” is a state of it. The possibility of turning into competence state is proportional to the amount of comK. We can substitute the protein involved in competence state for the protein we need. | + | 1) comK is a protein produced by bacillus subtilis and “competence” is a state of it. The possibility of turning into competence state is proportional to the amount of comK. We can substitute the protein involved in competence state for the protein we need.<br> |
| - | 2) The system can be used as a special lock. | + | 2) The system can be used as a special lock.<br> |
| - | 3) How to make circuit one recover its function as quick as possible? | + | 3) How to make circuit one recover its function as quick as possible?<br> |
| - | a) by means of protease? | + | a) by means of protease?<br> |
| - | + | b) by means of aptamer?<br> | |
| - | II. Set up groups to continue brainstorm | + | <br> |
| - | + | II. Set up groups to continue brainstorm<br> | |
| - | III. Make plans for the whole term. | + | <br> |
| + | III. Make plans for the whole term.<br> | ||
</p> | </p> | ||
Revision as of 08:04, 20 September 2012
GROUP MEETINGS
18th, Feb
Place: Room 363, Life Science building
Instructor: Mr.Hong
Recordist: Wuyang Chen
I. Discuss about our project:
1. SNS:
To complete this project is hardly possible. While it is likely to realize part of it, such as a system which is able to create and sense signals. But we need to confer our project some new meanings.
1) About making a signal disappear, according to Mr. Hong, we can add a rapid degradation label onto the signal.
2) We are afraid that the colony is too small to create an appropriate concentration gradient. According to Mr. Hong, it won’t be a big problem.
2. Solve the graph colouring problem using E.coli
1) In fact, we will only use E.coli to emit light in different colors, and all the algorithm for solving graph coloring problem is processed artificially.
2) We need to consult professors in Chemistry Department about constructing structures using ssDNA.
3) The scale of DNA strand is far too small than that of E.coli.
4) The fusion protein of Zn finger and the Ag43 would be very useful. We can think about how to make use of it.
3. Fatigue of specific stimulus
1) comK is a protein produced by bacillus subtilis and “competence” is a state of it. The possibility of turning into competence state is proportional to the amount of comK. We can substitute the protein involved in competence state for the protein we need.
2) The system can be used as a special lock.
3) How to make circuit one recover its function as quick as possible?
a) by means of protease?
b) by means of aptamer?
II. Set up groups to continue brainstorm
III. Make plans for the whole term.
July 3-July 9
7.3
extract the plasmids from the reproduced bacteria and send it to check the base sequence result: the concentration of the plasmids extracted is about 500ng/uL
7.4
extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2010 and put them into Top10 strain by transformation (LuxPr: Plate 2,24C Terminator : Plate 1,6O) result: the bacteria on the plate with Amp resistency grows well 7.5
pick up single colony from the Petri dish to reproduce in the liquid culture medium
7.6
extract plasmids containing LuxPR and Terminator and make enzyme digestion with EcoR1 and Spe1 in order to validate the existence of the two parts recieve part Toggle Switch and rbs-Ci-ter from PKU and conduct transformation
result: Due to the short length of LuxPR and Terminator, the result of the enzyme digestion is hard to examine by electrophoresis.
7.7
result: there are a few colonies with part Toggle switch grown while no colony of rbs-Ci-ter appeared.
7.8
pick up single colony with part Toggle switch . extract the standard parts LuxPR and Terminator from plate sent by iGEM registry 2011 and put them into Top10 strain by transformation
7.9
pick up single colony with LuxPR & terminator. extract plasmids containing toggle switch to conduct PCR.
process PCR with plasmids aptamer-CheZ result:as the picture shows. the base sequence of CheZ extracted before is proved right.
July 10-July 16
7.10
ligate aptamer-CheZ with PSB1C3 plasmids
extract plasmids containing LuxPR and terminator result:the concentractions of plasmids LuxPR and terminator are both 600ng/ul
7.11
transform PSB1C3 containing aptamer-CheZ into bacteria and cultivate. 7.12
carry out Double digestion of toggle switch and pSB1c3 and ligate them together.
pick up single colony from bacteria cultivated yesterday.
result: Both the location and the brightness of the electrophoretic bands correspond with our expectation.
7.14
transform the part rbs-ci-ter sent from PKU again into bacteria. check the colors of the colonies containing toggle switch. result: the ratio of red vs green is 8:25, verifying the function of toggle switch.
7.15
pick up the single colony from the plate yesterday and ten hours later extract the plasmids containing Ci-terminator.
7.16
conduct PCR with the plasmids yesterday to reproduce rbs-ci-Term. result:failure
