Team:USTC-China/parts

Four categories for our parts

1. Testing the characterization of the modified promoter pRM being

activated by the protein CI2.

BBa_K741001: plac-RBS-cI-T

BBa_K741002: plac-RBS-GFP-T

BBa_K741003: pRM-RBS-GFP-T

BBa_K741004: pRM-RBS-GFP-T-plac-RBS-cI-T

2. Testing the ability of the anticro to repress the

expression of the fusion protein crogfp.

BBa_K741000: antisense RNA of cro

BBa_K741005: Fusion Protein: crogfp

BBa_K741006: RBS-crogfp-T

BBa_K741007: plac-RBS-crogfp-T

BBa_K741008: pCon(0.244)-RBS-crogfp-T

BBa_K741009: pCon(0.856)-RBS-crogfp-T

BBa_K741010: plac-anticro-T

BBa_K741011: plac-anticro-T-pCon(0.244)-RBS-crogfp-T

BBa_K741012: plac-anticro-T-pCon(0.856)-RBS-crogfp-T

BBa_K741016: pRM-anticro-T

BBa_K741017: plac-RBS-cI-T-pCon(0.244)-RBS-crogfp-T-pRM-anticro-T

BBa_K741018: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T-pRM-anticro-T

BBa_K741020: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T

BBa_K741021: plac-RBS-cI-T-pRM-anticro-T

3. Testing the effect of the basic level of expression of

protein Lysis on the growth of bacteria.

BBa_K741013: RBS-lysis-T

BBa_K741014: pRM-RBS-lysis-T

BBa_K741015: plac-RBS-cI-T-pRM-RBS-lysis-T

.

4. Our final system.

BBa_K741019: pRM-anticro-T-pRM-RBS-lysis-T evaluate the lysis; the final results

Favorite:

BBa_K741001: plac-RBS-cI-T

BBa_K741002: plac-RBS-GFP-T

BBa_K741003: pRM-RBS-GFP-T

BBa_K741004: pRM-RBS-GFP-T-plac-RBS-cI-T

BBa_K741006: RBS-crogfp-T

BBa_K741008: pCon(0.244)-RBS-crogfp-T

BBa_K741009: pCon(0.856)-RBS-crogfp-T

BBa_K741010: plac-anticro-T

BBa_K741011: plac-anticro-T-pCon(0.244)-RBS-crogfp-T

BBa_K741012: plac-anticro-T-pCon(0.856)-RBS-crogfp-T

BBa_K741014: pRM-RBS-lysis-T

BBa_K741015: plac-RBS-cI-T-pRM-RBS-lysis-T

BBa_K741016: pRM-anticro-T

BBa_K741018: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T-pRM-anticro-T

BBa_K741019: pRM-anticro-T-pRM-RBS-lysis-T evaluate the lysis; the final results

BBa_K741020: pCon(0.856)-RBS-crogfp-T-plac-RBS-cI-T

BBa_K741021: plac-RBS-cI-T-pRM-anticro-T

New BioBrick part:

Natural:


  the best part:



• BBa_K741019: pRM-anticro-T-pRM-RBS-lysis-T evaluate the lysis; the final results

  other parts:


• BBa_K741000: antisense RNA of cro
• BBa_K741005: Fusion Protein: crogfp
• BBa_K741006: RBS-crogfp-T
• BBa_K741007: plac-RBS-crogfp-T
• BBa_K741008: pCon(0.244)-RBS-crogfp-T
• BBa_K741009: pCon(0.856)-RBS-crogfp-T
• BBa_K741010: plac-anticro-T
• BBa_K741011: plac-anticro-T-pCon(0.244)-RBS-crogfp-T
• BBa_K741012: plac-anticro-T-pCon(0.856)-RBS-crogfp-T
• BBa_K741016: pRM-anticro-T
• BBa_K741017: plac-RBS-cI-T-pCon(0.244)-RBS-crogfp-T-pRM-anticro-T
• BBa_K741018: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T-pRM-anticro-T
• BBa_K741020: plac-RBS-cI-T-pCon(0.856)-RBS-crogfp-T
• BBa_K741021: plac-RBS-cI-T-pRM-anticro-T





Engineered:


  the best part:



• BBa_K741015: plac-RBS-cI-T-pRM-RBS-lysis-T

  other parts:



• BBa_K741001: plac-RBS-cI-T
• BBa_K741002: plac-RBS-GFP-T
• BBa_K741003: pRM-RBS-GFP-T
• BBa_K741004: plac-RBS-cI-T-pRM-RBS-GFP-T
• BBa_K741013: RBS-lysis-T
• BBa_K741014: pRM-RBS-lysis-T



Best improved part(s):





• BBa_K741015: plac-RBS-cI-T-pRM-RBS-lysis-T
• BBa_K741004: plac-RBS-cI-T-pRM-RBS-GFP-T
• BBa_K741003: pRM-RBS-GFP-T
• BBa_K741014: pRM-RBS-lysis-T