Team:Goettingen/week21-2

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Revision as of 10:43, 18 September 2012

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V09_17_1 Preparative double digestion of flhDC, FliC, 18K-RFP and pSB1C3 followed by ligation

Experiment:
In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3, all components were digested with EcoRI and PstI according to the protocol. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .

V09_17_2 Preparation of over night cultures

V09_18_1 Motility Assays

V09_18_2 Preparative double digestion of MotA, MotB, yhjH, 18C-RFP, 20E-RFP and 20I-RFP followed by ligation

V09_18_3 Chemical transformation of

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <li>Experiment: <br>
 In order to clone the <i>flhDC</i>, <i>FliC</i> and 18K-<i>RFP</i> constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
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 <b>V09_17_2 Preparation of over night cultures</b><br>
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 <li>Experiment: <br>
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+<b>V09_18_2 Preparative double digestion of <i>MotA</i>, <i>MotB</i>, <i>yhjH</i>, 18C-<i>RFP</i>, 20E-<i>RFP</i> and 20I-<i>RFP</i> followed by ligation</i></b><br>
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+<li>Experiment: <br>
+TEXT<br>
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+<b>V09_18_3 Chemical transformation of</b><br>
+<ul>
+<li>Experiment: <br>
+TEXT<br>
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