Team:Goettingen/week8-3

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Revision as of 11:11, 17 September 2012

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Isolation of vector backbone pSB1C3 from BioBrick distribution kit

Experiment:
The vector pSB1C3 is used as the standard vector for BioBricks in this year's iGEM competition. We transformed E. coli DH10B with pSB1C3 according to protocol and streaked these out on Chloramphenicol (CM) plates.

Preparative digest of promoter_TAR_QC constructs and pSB1C3

Experiment:
All 8 promoter constructs + TAR_QC in pUC18 and the BioBrick vector pSB1C3 were digested according to protocol.

Observations and results:
The transformation of DH10B with pSB1C3 (see V06_18) worked well.

Preparative digest of promoter_TAR_QC constructs and pSB1C3

Experiment:
All 8 promoter constructs + TAR_QC in pUC18 and the BioBrick vector pSB1C3 were digested according to protocol.

Observations and results:
The transformation of DH10B with pSB1C3 (see V06_18) worked well.

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <td width="900" bordercolor="black" valign="top">
 <h2><b>V06_21 </b></h2><br>
+<b>Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
+<ul>
+<li>Experiment: <br>All 8 promoter constructs + TAR_QC in pUC18 and the BioBrick vector pSB1C3 were digested according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol.</a></li>
+</ul>
+<ul>
+<li>Observations and results: <br>The transformation of DH10B with pSB1C3 (see V06_18) worked well.
+</li>
+</ul>
+<br>
 <b>Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
 <ul>