"
Team:TU Darmstadt/Protocols
From 2012.igem.org
(Difference between revisions)
(→DNA) |
(→DNA) |
||
| Line 77: | Line 77: | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium sulfate precipitation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium sulfate precipitation] | ||
| - | |||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation] | ||
Revision as of 22:31, 16 September 2012
Protocols
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.
Contents |
In vivo
[http://en.wikipedia.org/wiki/E._coli Escherichia coli]
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- In-fusion BioBrick assembly
- Miniprep / Fermentas kit
- Miniprep / Promega kit
- Miniprep / Qiagen kit
- Protein Expression Est13
- Protein Expression FsC
- Transformation of chemically competent cells
- Western blot
Comamonas testosteroni
- [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
- ...
In vitro
Nucleic acids
[http://en.wikipedia.org/wiki/DNA DNA]
- Agarose gel electrophoresis
- Ammonium sulfate precipitation
- Cloning
- DNA Ligation
- DNA Quantification / NanoDrop
- DNA Synthesis from Oligos
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Miniprep
- PCR
- Polyacrylamide gel electrophoresis
- Primer Design
- Purification of DNA
- Restriction digest
- Resuspension of primers
- Salting Out
- Sequencing DNA
[http://en.wikipedia.org/wiki/RNA RNA]
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
In silico
Data analysis
Databases
Modelling
Sequence analysis
Structure analysis
Material Science
Miscellaneous
Cell culture
Microscopy
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
...3 Uhr ich geh ins Bett, mal wieder ...
