Team:Goettingen/week20-2

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Revision as of 13:05, 16 September 2012

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V09_10_1 Mini Prep of Psb1c3 (including motA, MotB or yhjH)

V09_10_2 Test digestion of Psb1c3 (including motA, motB or yhjH)

Experiment:
Test digestion of Psb1c3 (including motA, yhjH or motB) with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.

V09_10_3 Digestion of motA, motB, yhjH and the promoters 20E, 20I and 18C

Experiment:
MotA, MotB and yhjH were digested with XbaI and PstI. The plasmids with the different promoters were treated with SpeI and PstI. Digestion was controlled via gel-electrophoresis.The expected bands were isolated from the gel and purified via PeqGOLD Gelextraction Kit (Peqlab).

V09_10_4 Quickchange of FliC

Experiment:
Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols").No PCR product was observed

V09_11_1 Ligation and Transformation of motA, motB and yhjH

Experiment:
After the ligation of motA, motB and yhjH into the plasmid J61002 with different promoters (20E, 20I and 18C), the ligation products were transformed into the E. coli strain DH10B.

V09_11_2 Quickchange of FliC part 1

V09_12_1 Quickchange of FliC part 2

V09_12_2 Restriction and Ligation of promoter-RFP constructs into Psb1c3

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <h2><b>V09_10 </b></h2><br>
-<b>V09_10_1 Mini Prep of Psb1c3 (including motA, MotB and yhjH)</b><br>
+<b>V09_10_1 Mini Prep of Psb1c3 (including motA, MotB or yhjH)</b><br>
 <ul>
 <li>Experiment: <br>Plasmids were prepped using PeqGOLD MiniPrep Kit (Peqlab)</li>