Team:Goettingen/week21-2

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<h2><b>Versuchsnummer </b></h2><br>
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<h2><b>V09_17 </b></h2><br>
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<b>TITEL</i></b><br>
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<b>Preparative double digestion of flhDC, FliC, 18K-RFP and Psb1c3 followed by ligation</i></b><br>
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<li>Experiment: <br>  
<li>Experiment: <br>  
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TEXT.<br>
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In order to clone the flhDC, FliC and 18K-RFP constructs into pSB1C3,  all components were digested with EcoRI and PstI according to the <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. The restriction products were subsequently separated on a gel in order to verify the digestions success and purify the desired fragments. After measuring the concentration of the purified fragments, an overnight ligation was initiated .<br>
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Revision as of 07:22, 18 September 2012

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