Talk:Team:Columbia-Cooper-NYC/trial
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+ | |||
+ | </p><h2>Light Sensitive Spatially Controlled Micromachining of Copper Wafers Using Acidithiobacillus Ferrooxidans</h2> | ||
+ | <p>The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we intend to install a light sensitive mechanism which will enable us to etch copper in a desired pattern, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light will be focused on it in a desired pattern. The light sensitive mechanism in ferrooxidans would activate and self-destruct in the pathway of the light. In the end, the circuit board will be "etched" by the bacteria everywhere but the illuminated spots, leaving the desired pattern behind on the circuit board.</p> | ||
+ | |||
+ | <!--<p>The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we intend to install a light sensitive mechanism which will enable us to etch copper in a desired pattern, leaving a finished circuit board.</p>--> | ||
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Revision as of 14:29, 22 September 2012
Team:Columbia-Cooper-NYC
From 2012.igem.org
Light Sensitive Spatially Controlled Micromachining of Copper Wafers Using Acidithiobacillus Ferrooxidans
The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we intend to install a light sensitive mechanism which will enable us to etch copper in a desired pattern, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light will be focused on it in a desired pattern. The light sensitive mechanism in ferrooxidans would activate and self-destruct in the pathway of the light. In the end, the circuit board will be "etched" by the bacteria everywhere but the illuminated spots, leaving the desired pattern behind on the circuit board.
We placed genes from fireflies and bioluminescent bacteria into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative biosensors and biological alternatives to conventional lighting.
If you want a break from hard-core science, check out our Gibson Assembly music video.
You can also see view videos of our bacterial bubble lamp and project overview.