Team:Goettingen/week18-2

From 2012.igem.org

(Difference between revisions)
Line 538: Line 538:
<b>Transformation of FlhDC-contructs into E.coli strain Mg </b><br>
<b>Transformation of FlhDC-contructs into E.coli strain Mg </b><br>
<ul>
<ul>
-
<li>Experiment: <br>Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li>
+
<li>Experiment: <br>
 +
TEXT</li>
</ul>
</ul>
<br></td></tr>
<br></td></tr>
Line 546: Line 547:
<tr bordercolor="black" valign="top">
<tr bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
-
<h2><b>V09_04 </b></h2><br>
+
<h2><b>V08_28 </b></h2><br>
-
<b>V09_04_1 Test digestion of Psb1c3(including FlHDC under the control of different promoters) with</b><br>
+
<b>Restriction of flhDC-promoter constructs and Psb1c3 </b><br>
<ul>
<ul>
-
<li>Experiment: <br>Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li>
+
<li>Experiment: <br>
-
</ul>
+
TEXT</li>
-
<br>
+
-
<b>V09_04_2 Biobrick Standardization of motA, motB and yhjH</b><br>
+
-
<ul>
+
-
<li>Experiment: <br> PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).</li>
+
</ul>
</ul>
<br></td></tr>
<br></td></tr>
Line 562: Line 559:
<tr bordercolor="black" valign="top">
<tr bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
-
<h2><b>V09_05 </b></h2><br>
+
<h2><b>V08_29 </b></h2><br>
-
<b>V09_05_1 Double Digest of motA, motB, yhjH and Psb1c3</b><br>
+
<b>Ligation and transformation of flhDC-promoter constructs and Psb1c3 </b><br>
-
<ul>
+
-
<li>Experiment: <br> MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).</li>
+
-
</ul>
+
-
<br>
+
-
<b>V09_05_2 Quickchange of FliC</b><br>
+
<ul>
<ul>
-
<li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li>
+
<li>Experiment: <br>
 +
TEXT</li>
</ul>
</ul>
<br></td></tr>
<br></td></tr>
Line 578: Line 571:
<tr bordercolor="black" valign="top">
<tr bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
<td width="900" bordercolor="black" valign="top">
-
<h2><b>V09_06 </b></h2><br>
+
<h2><b>V08_30 </b></h2><br>
-
<b>Ligation and Transformation of motA, motB and yhjH</b><br>
+
<b>Mini prep </b><br>
<ul>
<ul>
-
<li>Experiment: <br>After the ligation of motA, motB and yhjH into the plasmid Psb1c3, the ligation products were transformed into the E. coli strain DH10B.</li>
+
<li>Experiment: <br>
 +
TEXT</li>
</ul>
</ul>
<br></td></tr>
<br></td></tr>

Revision as of 11:39, 15 September 2012

Retrieved from "http://2012.igem.org/Team:Goettingen/week18-2"