Team:Goettingen/week9-2

V06_25

• Experiment:
  In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol.
• Observations & Results:
  The transformation failed. Only the positive control featured colony formation. We found out that accidentally ampicillin containing plates were used whereas the plasmid obtains a chloramphenicol resistance. Thus, the out come is not surprising.

• Experiment:
  Since we received a new E. coli (DH10B) strain, over night cultures were prepared in order to produce new competent cells.The following protocol was considered.

V06_26

• Experiment:
  The preparation of the competent cells was performed according to the following protocol.
• Observations & Results:
  The performance of a test transformation proved the functionality of the new competent cells.

• Experiment:
  In order to test different E. coli strains competent cells of the recently received strains BL21, DH5alpha and XL1 Blue should be produced. On that account over night cultures of al strains were prepared.

V06_27

• Experiment:
  The preparation of the competent cells was performed according to the following protocol.

• Experiment:
  In order to test different E. coli strains competent cells of the recently received strains BL21, DH5alpha and XL1 blue should be produced. On that account over night cultures of al strains were prepared.

V06_20

• Experiment:
  Over night cultures were prepared for group 1 and group 3:
  
  20E-flhDC
  18M-flhDC
  18C-flhDC
  
  20E-tar
  18M-tar
  19C-tar