Team:Goettingen/week9-2

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Chemical transformartion of the BioBrick plasmid pSB1C3 into E. coli (DH10B)

Experiment:
In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol.
Observations & Results:
The transformation failed. Only the positive control featured colony formation. We found out that accidentally ampicillin containing plates were used whereas the plasmid obtains a chloramphenicol resistance. Thus, the out come is not surprising

Preparation of over night cultures

Experiment:
Since we received a new E. coli (DH10B) strain, over night cultures were prepared in order to produce new competent cells.
Observations & Results:

V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into E. coli (DH10B)

Experiment:
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.
Observations & Results:
The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.

V06_19_2 Repetition of the chemical transformartion of the araC-promoter into E. coli (DH10B)

Experiment:
Since the last time the fact that the araC-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol.
Observations & Results:
The transformation was again not successful. Only the positive control showed any growth.

Preparation of over night cultures

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

@@ Line 531: / Line 531: @@
 <td width="900" bordercolor="black" valign="top">
 <h2><b>V06_25 </b></h2><br>
-<b>Preparation of over night cultures</i></b><br>
+<b>Chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B)</i></b><br>
 <ul>
 <li>Experiment: <br>
-Since we received a new <i>E. coli</i> (DH10B) strain, over night cultures were prepared in order to produce new competent cells.<br>
+In order to gain further plasmid material of the new vector pSB1C3 was transformed into E. coli(DH10B) as described in the protocol. <br>
 <li>Observations & Results: <br>
+The transformation failed. Only the positive control featured colony formation. We found out that accidentally ampicillin containing plates were used whereas the plasmid obtains a chloramphenicol resistance. Thus, the out come is not surprising
 </li>
 </table>
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 <tr bordercolor="black" valign="top">
 <td width="900" bordercolor="black" valign="top">
-<h2><b>V06_25 </b></h2><br>
+<h2><b>V06_26 </b></h2><br>
 <b>Preparation of over night cultures</i></b><br>
 <ul>