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Team:Goettingen/week9-2
From 2012.igem.org
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| - | <h2><b><a name="week8#2">#2 Speed Improvement - | + | <h2><b><a name="week8#2">#2 Speed Improvement - 8th week</a></b></h2> |
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | ||
<br> | <br> | ||
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| - | <h2><b> | + | <h2><b>V06_25 </b></h2><br> |
| - | <b> | + | <b>Preparation of over night cultures</i></b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | + | Since we received a new <i>E. coli</i> (DH10B) strain, over night cultures were prepared in order to produce new competent cells.<br> | |
| - | <br> | + | <li>Observations & Results: <br> |
</li> | </li> | ||
</table> | </table> | ||
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| - | <h2><b> | + | <h2><b>V06_19 </b></h2><br> |
| - | <b> | + | <b>V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B) </b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | + | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li> | |
| + | <li>Observations & Results: <br> | ||
| + | The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed. | ||
</li></ul> | </li></ul> | ||
<br> | <br> | ||
| + | <b>V06_19_2 Repetition of the chemical transformartion of the <i>araC</i>-promoter into <i>E. coli</i> (DH10B) </b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> | ||
| + | Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol. <br> | ||
| + | <li>Observations & Results: <br> | ||
| + | The transformation was again not successful. Only the positive control showed any growth. </li> | ||
| + | </ul><br> | ||
| + | |||
</td></tr> | </td></tr> | ||
</table> | </table> | ||
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| - | <h2><b> | + | <h2><b>V06_20 </b></h2><br> |
| - | <b> | + | <b> Preparation of over night cultures</i></i></b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | + | Over night cultures were prepared for group 1 and group 3:<br> | |
| + | <br> | ||
| + | 20E-<i>flhDC</i> <br> | ||
| + | 18M-<i>flhDC</i> <br> | ||
| + | 18C-<i>flhDC</i> <br> | ||
| + | <br> | ||
| + | 20E-<i>tar</i> <br> | ||
| + | 18M-<i>tar</i> <br> | ||
| + | 19C-<i>tar</i> <br> | ||
<br></td></tr> | <br></td></tr> | ||
</table> | </table> | ||
Revision as of 10:43, 15 September 2012
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#2 Speed Improvement - 8th weekBack to overview
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