Team:Goettingen/week9-2

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<h2><b><a name="week8#2">#2 Speed Improvement - 8th week</a></b></h2>
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<h2><b><a name="week8#2">#2 Speed Improvement - 9th week</a></b></h2>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br>
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<h2><b>V06_25 </b></h2><br>
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<h2><b>Versuchsnummer </b></h2><br>
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<b>Preparation of over night cultures</i></b><br>
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<b>TITEL</i></b><br>
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<li>Experiment: <br>  
<li>Experiment: <br>  
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Since we received a new <i>E. coli</i> (DH10B) strain, over night cultures were prepared in order to produce new competent cells.<br>
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TEXT.<br>
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<li>Observations & Results: <br>
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<h2><b>V06_19 </b></h2><br>
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<h2><b>Versuchsnummer </b></h2><br>
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<b>V06_19_1 Repetition of the chemical transformartion of the BioBrick plasmid pSB1C3 into <i>E. coli</i> (DH10B) </b><br>
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<b>TITEL</b><br>
<ul>
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<li>Experiment: <br>
<li>Experiment: <br>
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Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual.</li>
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Text</li>
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<li>Observations & Results: <br>
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The transformation was successful.Due to the possesion of a gene encoding for RFP the developed colonied featured a red color. On the negative control no colonied were observed.
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<b>V06_19_2 Repetition of the chemical transformartion of the <i>araC</i>-promoter into <i>E. coli</i> (DH10B) </b><br>
 
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<li>Experiment:  <br>
 
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Since the last time the fact that the <i>araC</i>-promoter I0500 (14N) is inducible by ITPG was not considered, this time the substance was added to the LB-Medium (end concentration 100 µM). This is the only deogation from the standard transformation protocol. <br>
 
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<li>Observations & Results: <br>
 
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The transformation was again not successful. Only the positive control showed any growth.  </li>
 
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<h2><b>V06_20 </b></h2><br>
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<h2><b>Versuchsnummer </b></h2><br>
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<b> Preparation of over night cultures</i></i></b><br>
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<b> Titel</i></i></b><br>
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<li>Experiment: <br>  
<li>Experiment: <br>  
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Over night cultures were prepared for group 1 and group 3:<br>
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Text<br>
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Revision as of 10:41, 15 September 2012

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