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Team:Cambridge/Lab book/Week 14
From 2012.igem.org
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===Monday (24/09/12)=== | ===Monday (24/09/12)=== | ||
| + | The sensitivity of the sensor was tested using a dilution series of luciferase-producing bacteria. 20ml Cultures were grown overnight from single colonies. The cultures were induced with 40ul of 1.5M arabinose (for a final concentration of 3mM). Cultures were left for 2 1/2 hours for full induction. Subsequently, a culture was pelleted and resuspended in 4ml LB. Doubling dilutions, of volume 2ml, were made from this concentrate, down to 1/8th concentration. | ||
| + | 1ml of each 2ml dilution was analysed in each cuvette, which was placed in the cuvette holder we made ourselves. The result was very good. An almost linear relationship was obtained when data were normalised with the sensor value taken in the dark room without using the cuvette holder (1-(sensor value/sensor value in absolute dark)), presenting the sensitivity of the sensor to different intensities of light. | ||
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| + | [[File:Norm_light.png|thumb|400px|Normalised sensor data using a dilution series of bioluminescent E.Coli- Concentrations on the x-axis are relative therefore an OD 600 value was also taken|center]] | ||
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| + | [[File:Norm_blue.jpg|thumb|400px|Normalised percentage of blue frequencies using the same dilution series of E.Coli|center]] | ||
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| + | [[File:Dilution_series.jpg|thumb|400px|Dilution series of E.Coli|center]] | ||
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| + | Test of our circuitry in correctly identifying different frequencies (colours) of light. As can be seen below, measurements taken from orange and blue light yield values respectively above and below those from white light (our reference point). The data was taken using a constant intensity of light for each case (V.High and V.Low brightness). This was done with the aid of an Android phone and a specialised software application, called Color Flashlight, downloaded from the official Market. | ||
| + | [[File:Sensor_colour.jpgâ|400px|Sensor data for different colours at different intensities|thumb|center]] | ||
===Tuesday (25/09/12)=== | ===Tuesday (25/09/12)=== | ||
Revision as of 11:51, 25 September 2012
| Week: | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 |
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Contents |
Monday (24/09/12)
The sensitivity of the sensor was tested using a dilution series of luciferase-producing bacteria. 20ml Cultures were grown overnight from single colonies. The cultures were induced with 40ul of 1.5M arabinose (for a final concentration of 3mM). Cultures were left for 2 1/2 hours for full induction. Subsequently, a culture was pelleted and resuspended in 4ml LB. Doubling dilutions, of volume 2ml, were made from this concentrate, down to 1/8th concentration. 1ml of each 2ml dilution was analysed in each cuvette, which was placed in the cuvette holder we made ourselves. The result was very good. An almost linear relationship was obtained when data were normalised with the sensor value taken in the dark room without using the cuvette holder (1-(sensor value/sensor value in absolute dark)), presenting the sensitivity of the sensor to different intensities of light.
Test of our circuitry in correctly identifying different frequencies (colours) of light. As can be seen below, measurements taken from orange and blue light yield values respectively above and below those from white light (our reference point). The data was taken using a constant intensity of light for each case (V.High and V.Low brightness). This was done with the aid of an Android phone and a specialised software application, called Color Flashlight, downloaded from the official Market.
Tuesday (25/09/12)
Wednesday (26/09/12)
Thursday (27/09/12)
Friday (28/09/12)
