Team:Goettingen/week7-3

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Revision as of 10:51, 14 September 2012

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Insertion of TAR_QC into BioBricks plasmids

Preparation of J61002_promoter_TAR_QC

Experiment:
All 8 promoter constructs + TAR_QC were prepped using PeqGOLD Plasmid Miniprep kit (PeqLab) following the user manual. We performed a test digestion using XbaI and PstI to verify that the insert TAR_QC was successfully integrated into the plasmid. Glycerol stocks (30 %) of E. coli Δ tar J61002_promoter_TAR_QC were prepared and stored at -80 °C.

Observations and Results:
All eight constructs were successfully generated!

Important pages:
Home; Team; Official Team Profile; Project; Parts submitted to the Registry; Modeling; Notebook; Saftey; Attributions

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 <b>Preparation of J61002_promoter_TAR_QC</b><br>
 <ul>
-<li>Experiment: <br>All 8 promoter constructs + TAR_QC were prepped using PeqGOLD Plasmid Miniprep kit (PeqLab) following the user manual. We performed a test digestion using <i>Xba</i>I and <i>Pst</i>I to verify that the insert TAR_QC was successfully integrated into the plasmid.
+<li>Experiment: <br>All 8 promoter constructs + TAR_QC were prepped using PeqGOLD Plasmid Miniprep kit (PeqLab) following the user manual. We performed a test digestion using <i>Xba</i>I and <i>Pst</i>I to verify that the insert TAR_QC was successfully integrated into the plasmid. Glycerol stocks (30 %) of <i>E. coli</i> &Delta; <i>tar</i> J61002_promoter_TAR_QC were prepared and stored at -80 °C.
 </li>
 </ul>
+<li> Observations and Results: <br>All eight constructs were successfully generated! </li> </ul>
 <br></td></tr>
 </table>