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Team:Goettingen/week7-2
From 2012.igem.org
(Difference between revisions)
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<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
| - | In order to gain further plasmid material over night cultures of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br> | + | In order to gain further plasmid material over night cultures of one colonie of all eight <i>flhDC</i>-promoter constructs were prepared for subsequent plasmid isolation. <br> |
20E - #2 <br> | 20E - #2 <br> | ||
20G - #1 <br> | 20G - #1 <br> | ||
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<td width="900" bordercolor="black" valign="top"> | <td width="900" bordercolor="black" valign="top"> | ||
<h2><b>V06_13 </b></h2><br> | <h2><b>V06_13 </b></h2><br> | ||
| - | <b> | + | <b>V06_13_1 Miniprep of the new <i>flhDC</i>-promoter constructs</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. | Minipreps were conducted with PeqGOLD MiniPrep Kit (Peqlab) according to the user manual. | ||
<li> | <li> | ||
| - | |||
<br> | <br> | ||
| - | <b> | + | <b>V06_013_2 Chemical transformartion of pBAD-glGFP into E. coli (DH10B)</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> | <li>Experiment: <br> | ||
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<li>Observations & Results: <br> | <li>Observations & Results: <br> | ||
The transformation was successful since all plates showed numeours colonoes except the negative control.</li> | The transformation was successful since all plates showed numeours colonoes except the negative control.</li> | ||
| + | <br></td></tr> | ||
| + | </table> | ||
| + | |||
| + | <h2><b>V06_14 </b></h2><br> | ||
| + | <b>Preparation of over night cultures</i></b><br> | ||
| + | <ul> | ||
| + | <li>Experiment: <br> | ||
| + | Over night cultures of pBAD-glGFP were prepared in order to isolate the plasmids. <br> | ||
<br></td></tr> | <br></td></tr> | ||
</table> | </table> | ||
Revision as of 10:32, 14 September 2012
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Language:  English,  Deutsch
| #2 Speed Improvement - 7th weekBack to overview
V06_14Preparation of over night cultures
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