Team:Goettingen/week5-3

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<li>Experiment: <br>Transformation was carried out according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Transformed DH10B were plated on LB-Amp plates (see here for <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">recipe</a>). Transformation was successful.
<li>Experiment: <br>Transformation was carried out according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a>. Transformed DH10B were plated on LB-Amp plates (see here for <a href="https://2012.igem.org/Team:Goettingen/Project/Materials">recipe</a>). Transformation was successful.
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<h2><b>V05_31 </b></h2><br>
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<b>Preparation of pUC18_TAR</b><br>
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<li>Experiment: <br>Inocculation of overnight cultures of 10 supposedly positive clones in liquid LB-Amp (<a href="https://2012.igem.org/Team:Goettingen/Project/Materials">recipe</a>).
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<h2><b>V06_01 </b></h2><br>
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<b>Changing the <i>Xba</i>I site in the <i>tar</i> sequence via QuikChange</b><br>
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<li>Experiment: <br>DNA was prepped using PeqGOLD MiniPrep Kit (Peqlab). A DNA test digest with <i>Eco</i>RI and <i>Xba</i>I was performed according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol</a> and analyzed on a 1% agarose gel. Test digest showed 8 out of 10 clones contained the construct pUC18_TAR. QuikChange PCR was performed using designed primers and <i>Pfu</i> Turbo polymerase (see QuikChange protocol <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">here</a>).
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Revision as of 13:37, 12 September 2012

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