Team:UC Chile2/General Protocols

From 2012.igem.org

(Difference between revisions)
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<div id="Media">
<div id="Media">
-
 
== Media ==
== Media ==
</div>
</div>
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<h1>LB media
+
<h1>LB media</h1>
-
</h1>
+
<ul>
 +
<li>1% (w/v) tryptone</li>
 +
<li>0.5% (w/v) yeast extract</li>
 +
<li>86 mM NaCl</li>
 +
<h2>Per liter:</h2>
 +
<li>10g Tryptone</li>
 +
<li>5g Yeast extract</li>
 +
<li>5g NaCl</li>
 +
<li>15g Agar -Optional- /L</li>
 +
<li>Adjust pH to 7.5 with NaOH 1M</li>
 +
</ul>
 +
<h1>SOB media</h1>
<ul>
<ul>
-
<li>Tryptone 10gr/L</li>
+
<li>0.5% (w/v) yeast extract</li>
-
<li>Yeast extract 5gr/L</li>
+
<li>2% (w/v) tryptone</li>
-
<li>NaCl 5gr/L</li>
+
<li>10 mM NaCl</li>
-
<li>-Optional- Agar 15gr/L</li>
+
<li>2.5 mM KCl</li>
 +
<li>20 mM MgSO4</li>
 +
<h2>Per liter:</h2>
 +
<li>5 g yeast extract</li>
 +
<li>20 g tryptone</li>
 +
<li>0.584 g NaCl</li>
 +
<li>0.186 g KCl</li>
 +
<li>2.4 g MgSO4</li>
 +
<li>15g Agar -Optional-</li>
 +
<li>Adjust pH to 7.5 with NaOH 1M</li>
</ul>
</ul>

Revision as of 02:22, 12 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012