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Team:TU Darmstadt/Materials/XbaI
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Latest revision as of 20:48, 26 September 2012
Contents |
XbaI
About
XbaI is a endonucleasic restriction enzyme isolated from [http://en.wikipedia.org/wiki/Xanthomonas_badrii Xanthomonas badrii]. It creates stick ends with 5' end overhang.
Working conditions
The recognition motive of XbaI is:[1]
5' TCTAGA --> 5'---T CTAGA---3' 3' AGATCT --> 3'---AGATC T---5'
Buffer: NEBuffer 2 + BSA
SpeI requires at least 1bp overhang on its cutting site (99% cleavage efficiency).[2]
Supplier
- NEB http://www.neb.com/nebecomm/products/productR0145.asp
References
[1] http://en.wikipedia.org/wiki/List_of_restriction_enzyme_cutting_sites:_T-Z#X
[2] http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_linearized_vector.asp
