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Team:Goettingen/week19-2
From 2012.igem.org
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| - | <h2><b><a name="week3#2">#2 Speed Improvement - | + | <h2><b><a name="week3#2">#2 Speed Improvement - 18th week</a></b></h2> |
<a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | <a href="https://2012.igem.org/Team:Goettingen/Notebook">Back to overview</a><br> | ||
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| - | <h2><b> | + | <h2><b>V09_04 </b></h2><br> |
| - | <b>Test digestion of Psb1c3(including FlHDC under the control of different promoters) with</b><br> | + | <b>V09_04_1 Test digestion of Psb1c3(including FlHDC under the control of different promoters) with</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br>Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | <li>Experiment: <br>Test digestion of Psb1c3 including FlHDC under the expression of 8 different promoters with the restriction enzymes EcoRI and PstI. Test digest worked for all clones.</li> | ||
</ul> | </ul> | ||
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| - | + | <b>V09_04_2 Biobrick Standardization of motA, motB and yhjH</b><br> | |
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| - | <b>Biobrick Standardization of motA, motB and yhjH</b><br> | + | |
<ul> | <ul> | ||
<li>Experiment: <br> PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).</li> | <li>Experiment: <br> PCR of motA, motB and yhjH using primers containing the standard BioBrick sequences. Procedure was succesful and the PCR products were purified via PeqGOLD Gelextraction Kit (Peqlab).</li> | ||
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| - | <h2><b> | + | <h2><b>V09_05 </b></h2><br> |
| - | <b>Double Digest of motA, motB, yhjH and Psb1c3</b><br> | + | <b>V09_05_1 Double Digest of motA, motB, yhjH and Psb1c3</b><br> |
<ul> | <ul> | ||
<li>Experiment: <br> MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).</li> | <li>Experiment: <br> MotA, motB, yhjH and Psb1c3 were digested with EcoRI and PsT1. The prodcuts were seperated via gel electrophoresis and purified using PeqGOLD Gelextraction Kit (Peqlab).</li> | ||
</ul> | </ul> | ||
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| - | + | <b>V09_05_2 Quickchange of FliC</b><br> | |
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| - | <b>Quickchange of FliC</b><br> | + | |
<ul> | <ul> | ||
<li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li> | <li>Experiment: <br> Quick Change overlapping PCR was performed using designed primers and Pfu Turbo polymerase (see Quick Change protocol in "protocols"). Was not succesful. We Could not observed any PCR product.</li> | ||
</ul> | </ul> | ||
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<b>Ligation and Transformation of motA, motB and yhjH</b><br> | <b>Ligation and Transformation of motA, motB and yhjH</b><br> | ||
<ul> | <ul> | ||
| - | <li>Experiment: <br> After the ligation of motA, motB and yhjH into the plasmid Psb1c3, the ligation products were transformed into the E. coli strain DH10B.</li> | + | <li>Experiment: <br>After the ligation of motA, motB and yhjH into the plasmid Psb1c3, the ligation products were transformed into the E. coli strain DH10B.</li> |
</ul> | </ul> | ||
<br></td></tr> | <br></td></tr> | ||
Revision as of 11:29, 15 September 2012
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#2 Speed Improvement - 18th weekBack to overview
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