Team:UC Chile2/Bactomithril/Notebook

From 2012.igem.org

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The next day we did colony PCR of those transformed bacteria at a Tm=59ºC. From all the colonies that we used, only one of pBAD_ADF-3 in pSB1K3 and one of pBAD_ADF-3+ in pSB1K3 had the correct size. Then we left these bacteria growing, so that we could purificate their DNA later .
The next day we did colony PCR of those transformed bacteria at a Tm=59ºC. From all the colonies that we used, only one of pBAD_ADF-3 in pSB1K3 and one of pBAD_ADF-3+ in pSB1K3 had the correct size. Then we left these bacteria growing, so that we could purificate their DNA later .
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Latest revision as of 23:53, 21 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012