Team:Arizona State

From 2012.igem.org

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Diarrheic pathogens including E.coli O157:H7 serotype, campylobacter, shigella, and salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths.  Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization.  Our method of bacterial detection fills a niche in biosensor technology.  Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors.  Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection.  Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme.  This functioning enzyme unit then cleaves x-gal and produces a colorimetric output signal.  Our research demonstrates success in initial stages of chimeric protein assembly.   
Diarrheic pathogens including E.coli O157:H7 serotype, campylobacter, shigella, and salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths.  Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization.  Our method of bacterial detection fills a niche in biosensor technology.  Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors.  Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection.  Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme.  This functioning enzyme unit then cleaves x-gal and produces a colorimetric output signal.  Our research demonstrates success in initial stages of chimeric protein assembly.   
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<html>
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<h1>Website To Do List:</h1>
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<p><b>DO NOT DELETE THIS LIST!</b> may be used for "Site Map". Due October 3, <b><i>THREE WEEKS!!</i></b></p>
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<ul>
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<li>Project</li>
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<ul>
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<li>make "Home" page pretty</li>
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<li>add info to "Problem" page</li>
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<li>make "Overview" page pretty</li>
 +
<li>add info to "Magainin" page</li>
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<li>add info to "Chimeric Reporter" page</li>
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<li>add info to "ssDNA" page</li>
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</ul>
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<li>Team</li>
 +
<ul>
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<li>update bios on "Team" page</li>
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</ul>
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<li>Results</li>
 +
<ul>
 +
<li>Get Lab Data</li>
 +
<li>add info to "Problem" page</li>
 +
<li>add info to "Data" page</li>
 +
<li>add info to "Main Results" page</li>
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<li>add info to "Judging Criteria" page</li>
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<li>add info to "Regional Jamboree" page</li>
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</ul>
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<li>Human Practices</li>
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<ul>
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<li>approve "University" page?</li>
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<li>add info to "Community" page</li>
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<li>add info to "Regional Jamboree" page</li>
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<li>add info to "International" page</li>
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<li>make "Field Applications" page</li>
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<li>make "Modeling" page</li>
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</ul>
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<li>Extras</li>
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<ul>
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<li>approve "Safety" page?</li>
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<li>add info to "Site Map" page</li>
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</ul>
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</ul>
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</html>

Revision as of 15:44, 12 September 2012


Abstract

Diarrheic pathogens including E.coli O157:H7 serotype, campylobacter, shigella, and salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths. Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization. Our method of bacterial detection fills a niche in biosensor technology. Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors. Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection. Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme. This functioning enzyme unit then cleaves x-gal and produces a colorimetric output signal. Our research demonstrates success in initial stages of chimeric protein assembly.


Website To Do List:

DO NOT DELETE THIS LIST! may be used for "Site Map". Due October 3, THREE WEEKS!!

  • Project
    • make "Home" page pretty
    • add info to "Problem" page
    • make "Overview" page pretty
    • add info to "Magainin" page
    • add info to "Chimeric Reporter" page
    • add info to "ssDNA" page
  • Team
    • update bios on "Team" page
  • Results
    • Get Lab Data
    • add info to "Problem" page
    • add info to "Data" page
    • add info to "Main Results" page
    • add info to "Judging Criteria" page
    • add info to "Regional Jamboree" page
  • Human Practices
    • approve "University" page?
    • add info to "Community" page
    • add info to "Regional Jamboree" page
    • add info to "International" page
    • make "Field Applications" page
    • make "Modeling" page
  • Extras
    • approve "Safety" page?
    • add info to "Site Map" page

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