Team:UC Chile

From 2012.igem.org

(Difference between revisions)

Latest revision as of 22:46, 13 November 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012

Luxilla Biolamp takes advantage of one the most fascinating natural phenomena: bioluminescence.

We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.

Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.

We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.

Al being lit by bioluminescent bacteria!

@@ Line 1: / Line 1: @@
-{{UC_Chile}}
+{{UC_Chile4}}
+<html>
+		<div class="slider-wrapper theme-default">
+            <div class="ribbon"></div>
+            <div id="slider" class="nivoSlider">
+                <img src="https://static.igem.org/mediawiki/2012/7/7a/UC_Chile-Bioluminescence.jpg" alt="" title="#htmlcaption1"/>
+<img src="https://static.igem.org/mediawiki/2012/5/5a/UC_Chile-Syne.jpg" alt="" title="#htmlcaption2"/>
+<img src="https://static.igem.org/mediawiki/2012/f/fa/UC-Chile-Constructs.jpg" alt="" title="#htmlcaption4"/>
+<img src="https://static.igem.org/mediawiki/2012/1/1d/Syne_Transformation.JPG" alt="" title="#htmlcaption5"/>
+<img src="https://static.igem.org/mediawiki/2012/1/15/The_team_and_biolamp2.jpg" "/>
+            </div>
-<div id="Bacto">
-== Spider-Coli ==
+<div id="htmlcaption1" class="nivo-html-caption">
-</div>
+<center style="font-family:'Vollkorn', serif; color: white;">Luxilla Biolamp takes advantage of one the most fascinating natural phenomena: bioluminescence. </center></div>
+<div id="htmlcaption2" class="nivo-html-caption">
+<center style="font-family:'Vollkorn', serif; color: white;">We chose for the chassis the cyanobacteria Synechocystis PCC 6803, because it is a photoautotroph and is regulated by circadian rhythms.</center></div>
-[[File:SpiderSilk.jpg|center|960px|enlace=<http://www.flickr.com/photos/scoobygirl/94943906/lightbox/>]]
+<div id="htmlcaption4" class="nivo-html-caption">
+<center style="font-family:'Vollkorn', serif; color: white;">Using the lux operon and circadian promoters, we designed two plasmids: the Luciferase generator is to produce bioluminiscence, and the substrate generator recovers substainably the substrates necessary for the reactions.</center></div>
+<div id="htmlcaption5" class="nivo-html-caption">
+<center style="font-family:'Vollkorn', serif; color: white;">We achieved to transform Synechocystis with the luciferase generator plasmid, and verificated light emission in a luminometer.</center></div>
-<p> Our goal is to generate new synthetic spider silk-like proteins that maintain or improve some of the properties of the spider silk and to efficiently create a new biomaterial for biomedical or construction applications.
-</p>
+            <div id="htmlcaption" class="nivo-html-caption">
+<center style="font-family:'Vollkorn', serif; color: white;">Al being lit by bioluminescent bacteria! </center></div>
+		</div>
+</html>
 <div id="Cyano">
-== Cyanolux ==
+<html>
-[[File:SpiderSilk.jpg|center|960px|enlace=<http://cdn-live2.blackenterprise.net/wp-content/blogs.dir/1/files/2011/01/energy-efficient-light-bulb.jpg>]]
+<center>
+<table width=900 height=500 style=”border:none;”>
+<tr>
+<td><a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Project_short"><img src="https://static.igem.org/mediawiki/2012/f/f9/UC_Chile-Animated-abstract-final.gif" align="left">
+<img src="https://static.igem.org/mediawiki/2012/5/5f/UC_ChileSide-projects3_-_title.jpg" align="right">
+<a href="https://2012.igem.org/Team:UC_Chile/Bactomithril"><img src="https://static.igem.org/mediawiki/2012/d/d6/UC_ChileSide-projects3_-_bacto.jpg" align="right">
+<a href="https://2012.igem.org/Team:UC_Chile/Results/Gibson"><img src="https://static.igem.org/mediawiki/2012/0/02/UC_ChileSide-projects3_-_gibson.jpg" align="right">
+<a href="https://2012.igem.org/Team:UC_Chile/Results/LuxBrick"><img src="https://static.igem.org/mediawiki/2012/4/4e/UC_ChileSide-projects3_-_lux.jpg" align="right">
+<a href="https://2012.igem.org/Team:UC_Chile/Results/Growthcurve"><img src="https://static.igem.org/mediawiki/2012/a/a8/UC_CHileSide-projects3_-_growth.jpg" align="right"><a href="https://2012.igem.org/Team:UC_Chile/Results/Int_C"><img src="https://static.igem.org/mediawiki/2012/f/f1/UC_ChileSide-projects3_-_debugging.jpg" align="right"><a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Future"><img src="https://static.igem.org/mediawiki/2012/e/e9/UC_ChileSide-projects3_-_gfp.jpg" align="right"></td>
+</tr>
+</table>
+<center>
+</html>
+[[File:Results_jamboree_igem_uc_chile2.jpg]]
+<br><br>
-Synthethic biology aims to quantitatively design, control and predict complex spatiotemporal cell behavior, and to doing so by developing standarized and engineerable genetic tools, nevertheless, to the date, no synthetic genetic circuit has acomplished the task of exhibit a robust and constant (i.e phase and amplitude in a week period) time oscillation. We want to take advantage of more than 2000 million years of evolution of cyanobacteria to use their endogenous circadian rythm to develop a time-controlled metabolic pathway. As a proof of concept we want to engineer the first light-rechargeable biological lamp: Synechocystis pcc6803 cells  that emit light only by night while recovering and producing the substrates in the day.
-</div>
+{{UC_Chilefooter}}