Team:UIUC-Illinois/Notebook/Protocols/TAE
From 2012.igem.org
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Procedure:<br/><br/> | Procedure:<br/><br/> | ||
- | 1. In a 1000mL pyrex bottle add and mix: | + | 1. In a 1000mL pyrex bottle add and mix: <br/> |
- 242 Tris base<br/> | - 242 Tris base<br/> | ||
- 57.1mL glacial acetic acid<br/> | - 57.1mL glacial acetic acid<br/> | ||
- 37.2g Na2EDTA•2H2O<br/> | - 37.2g Na2EDTA•2H2O<br/> | ||
- | - 1000mL MilliQ water<br/> | + | - 1000mL MilliQ water<br/><br/> |
2. Mix thoroughly, no sterilization necessary<br/><br/> | 2. Mix thoroughly, no sterilization necessary<br/><br/> | ||
<h2>Making 1X TAE buffer</h2><br/> | <h2>Making 1X TAE buffer</h2><br/> | ||
- | What you need: <br/> | + | What you need: <br/><br/> |
- 50X TAE buffer <br/> | - 50X TAE buffer <br/> | ||
- MilliQ water <br/><br/> | - MilliQ water <br/><br/> |
Latest revision as of 15:31, 4 June 2012