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Team:UIUC-Illinois/Notebook/Protocols/GelElectrophoresis
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<center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/> | <center><h1>Making Gel Electrophoresis gel solutions</h1></center> <br/><br/> | ||
| - | < | + | <h2>To check PCR results</h2><br/> |
| - | What you need:<br/> | + | What you need:<br/><br/> |
- High-melt agarose<br/> | - High-melt agarose<br/> | ||
| - | - TAE buffer 1X<br/><br/> | + | - TAE buffer 1X<br/> |
| + | - Autoclaved container <br/><br/> | ||
| - | Procedure:<br/> | + | Procedure:<br/><br/> |
1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/> | 1. Add 3.2g high-melt agarose to 400 mL 1X TAE buffer (50 uL agarose gel, 250 mL 1X TAE buffer for the small gel box) <br/> | ||
| - | 2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved | + | 2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> |
3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/> | 3. BE CAREFUL AS LIQUID CAN BE SUPERHEATED<br/><br/> | ||
| - | < | + | <h2>To do a gel purification</h2><br/> |
| - | What you need:<br/> | + | What you need:<br/><br/> |
- Low-melt agarose<br/> | - Low-melt agarose<br/> | ||
| - | - TAE buffer 1X<br/><br/> | + | - TAE buffer 1X<br/> |
| - | Procedure:<br/> | + | - Autoclaved container <br/><br/> |
| + | Procedure:<br/><br/> | ||
1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/> | 1. Add 4.0g low-melt agarose to 400 mL 1X TAE buffer<br/> | ||
2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> | 2. Microwave flask or bottle (cap placed loosely if bottle) of mixed solution to dissolve gel in 1 minute increments until all agarose is dissolved<br/> | ||
Latest revision as of 15:32, 4 June 2012
