Team:UIUC-Illinois/Notebook/Protocols/Subculturing
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2. Incubate in the 37˚C warm room on a stirrer for 4-6 hours <br/><br/> | 2. Incubate in the 37˚C warm room on a stirrer for 4-6 hours <br/><br/> | ||
- | Note: Need to check the cells 2 hours after inoculation to test their optical density (OD). The best competent cells come from cells in exponential phase of their growth. Therefore an OD of 0.4-0.8 is desired and this happens after 2-4 hours of incubation. <br/> | + | Note: Need to check the cells 2 hours after inoculation to test their optical density (OD). The best competent cells come from cells in exponential phase of their growth. Therefore an OD of 0.4-0.8 is desired and this happens after 2-4 hours of incubation. <br/><br/> |
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Latest revision as of 15:31, 4 June 2012