Team:UIUC-Illinois

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<center><h1>Mission Statement</h1></center>
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<p>The ultimate goal of the University of Illinois 2012 iGEM team is to construct an RNA based protein scaffold to use in the optimization of multi-step enzymatic pathways like that involved in the production of Piceatannol from less complex molecules. We aim to improve the versatility and cost efficiency of biological production systems by use of our PUF project RNA scaffold and to prove its viability through the implementation of such a system in our PHAT project's Piceatannol production in E.Coli.</p>
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<p>The ultimate goal of the University of Illinois 2012 iGEM team is to construct an RNA-based PUF protein toolkit which can be utilized for the manipulation of gene expression. Customizable manipulation will involve gene silencing by specific RNA scission. Also, the PUF protein's properties are suited for optimizing multi-step enzymatic pathways. In this way, we aim to improve the versatility and cost efficiency of biological production systems. With a protein-based RNA-binding toolkit, researchers will be able to transmute the biobricked PUF for use in their scientific endeavors. In addition, we seek to standardize and characterize the nature of PUF. <br/><center><font size="5"><a href="https://2012.igem.org/Team:UIUC-Illinois/Project"> Click here to read our project abstract.</a></font></center></p><br/>
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<center><h2>Our Research Outside of PUF Itself</h2></center><br/>
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<p>Aside from researching the RNA specific affinity of PUF and its endonuclease fusion protein variants, we have also explored and tested other potential applications for PUF. Mainly, our ancillary projects directly related to PUF include our RNA scaffold and an <a href="https://2012.igem.org/Team:UIUC-Illinois/Project/Future/AssemblyLine">enzymatic assembly line</a> based on such a scaffold. Additionally, <a href="https://2012.igem.org/Team:UIUC-Illinois/Project/Future/Petrobrick">we have characterized</a> the 2011 University of Washington iGEM team's Petrobrick <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K590025">(BBa_K590025)</a>.<br/><br/>
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Outside the dry and wet labs, we have invested ourselves in a human practices project and public awareness and education about synthetic biology and science in general. To learn more, please visit <a href="https://2012.igem.org/Team:UIUC-Illinois/Outreach">our Outreach section</a>.
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<h1>PROJECT PUF</h1>
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        <p>This project is focused on creating and optimizing a construct for the production and customization of the PUM protein. In doing so, our team is looking to standardize the method in which the RNA binding protein can be used as a toolkit in Biobrick format. With a toolkit we hope to produce utility with biological parts while following the philosophies of engineering. This toolkit will then be used in various ways to further control distinct aspects of anything involving RNA. In doing so, we also seek to utilize such properties, zinc fingers, and RNA stem loops to create a protein scaffolding system based on RNA.</p>
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<h1>PROJECT PHAT</h1>
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        <p>The project dubbed "PHAT" has its focus on a metabolite of the widely studied chemical, Resveratrol. The metabolite of Resveratrol, Piceatannol has recently been discovered to have unique properties which can be heavily utilized in pharmaceutical industries as it has demonstrated an ability to inhibit adipogenesis. Our team seeks to integrate the disciplines of synthetic biology with aspects of chemical engineering to find efficient ways to produce and improve upon Piceatannol production. We hope to utilize the scaffolding system we will have developed to optimize the final results of project PHAT.</p>
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<center><p>Click above on a part of our project that you want to read about.</p></center></font>
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<center><h2><a href="https://2012.igem.org/Team:UIUC-Illinois/Project/Future/AssemblyLine">Enzymatic Assembly Line</a></h2><br/><center>
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<p>In utilizing the versatility of the PUF based toolkit we are developing, our current research involves an engineered metabolic pathway. The characterization and standardization of these genes in biobrick format is underway. Future goals involving the PUF toolkit will focus on tethering enzymes of productive artificial or natural pathways for kinetic favorability.</p>
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<center><h2><a href="https://2012.igem.org/Team:UIUC-Illinois/Project">PUF</a></h2><br/></center>
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<p>This is the PUF protein found on the PUM1 gene in humans. Our main focus and cause of interest in this protein is its ability to recognize single stranded RNA with specificity. The ability to modify PUF with tethered or fused proteins allows for the development of a customizable RNA binding toolkit. Depending on what fusion or tether is used, the functionality of the PUF protein is modified as well.</p>
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<center><h2><a href="https://2012.igem.org/Team:UIUC-Illinois/Project">RNA scission</a></h2><br/></center>
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<p>In fusion the PUF protein to a restriction endonuclease, the fusion protein demonstrates the ability to cleave single stranded RNA molecules with customizable specificity up to eight base pairs. Considering this ability, we believe PUF can prove itself as a promising new tool in researching and developing new types of gene therapy as it can silence genes with seqeunces specific to a reseachers interest.></p>
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<center><h2><a href="https://2012.igem.org/Team:UIUC-Illinois/Project/Future/Scaffold">RNA scaffold utilization</a></h2><br/></center>
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<p>Since PUF has affinity towards RNA molecules, it is possible to synthesize or utilize existing stemloop structures in RNA in order to provide a kinetically favorable platform for tethered proteins to interact. </p>
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<center><h2><font size="5"> Sponsors</font></h2></center>
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<a href="http://www.biotech.uiuc.edu/">
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Latest revision as of 08:17, 15 February 2013

Header

Main

Mission Statement

The ultimate goal of the University of Illinois 2012 iGEM team is to construct an RNA-based PUF protein toolkit which can be utilized for the manipulation of gene expression. Customizable manipulation will involve gene silencing by specific RNA scission. Also, the PUF protein's properties are suited for optimizing multi-step enzymatic pathways. In this way, we aim to improve the versatility and cost efficiency of biological production systems. With a protein-based RNA-binding toolkit, researchers will be able to transmute the biobricked PUF for use in their scientific endeavors. In addition, we seek to standardize and characterize the nature of PUF.

Click here to read our project abstract.


Our Research Outside of PUF Itself


Aside from researching the RNA specific affinity of PUF and its endonuclease fusion protein variants, we have also explored and tested other potential applications for PUF. Mainly, our ancillary projects directly related to PUF include our RNA scaffold and an enzymatic assembly line based on such a scaffold. Additionally, we have characterized the 2011 University of Washington iGEM team's Petrobrick (BBa_K590025).

Outside the dry and wet labs, we have invested ourselves in a human practices project and public awareness and education about synthetic biology and science in general. To learn more, please visit our Outreach section.

    • Click above on a part of our project that you want to read about.

    Sponsors


    goldbiologo genscriptlogo octochemlogo

    royjlogo laslogo

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