Team:Evry/Notebook/w6

From 2012.igem.org

(Difference between revisions)
(Created page with "{{:Team:Evry/template_v1}} <html> <h1>Weeks</h1> <table> <tr> <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td> <td><a href="https://2012.igem.org/Team:Evr...")
 
(One intermediate revision not shown)
Line 1: Line 1:
-
{{:Team:Evry/template_v1}}
+
{{:Team:Evry/template_notebook}}
<html>
<html>
-
 
-
<h1>Weeks</h1>
 
-
 
-
<table>
 
-
<tr>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w2">2</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w3">3</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w4">4</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w5">5</a></td>
 
-
  <td>6</td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w7">7</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w8">8</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w9">9</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w10">10</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w11">11</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w12">12</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w13">13</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w14">14</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w15">15</a></td>
 
-
  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w16">16</a></td>
 
-
</tr>
 
-
</table>
 
<h1>Week 6: 16th July - 22nd July</h1>
<h1>Week 6: 16th July - 22nd July</h1>

Latest revision as of 09:59, 3 August 2012

Weeks:

June July August September October November

Week 6: 16th July - 22nd July

Monday, 16th July

Bacterial Transformation:

Transformation of AID + TIR1 containing plasmid into TOP10 bacterias.

Thuesday, 17th July

Reception and preparation of pCS2+ primers.

Friday, 20th July

PCR Colony:

PCR colony of T10 pSC2+ with RFP
Primers used : SP6 and T3

Site Mutagenesis directed:

PCR for directed mutagenesis to replace 2 bases from TIR1 in order to remove 2 Restrictions Site
Primer used : SdMTIR1-Fw and SdMTIR1-Rv

Retrieved from "http://2012.igem.org/Team:Evry/Notebook/w6"