Team:NTNU Trondheim/Stocks

From 2012.igem.org

(Difference between revisions)
(→Agar plates)
 
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This page lists stored cultures and other samples:
This page lists stored cultures and other samples:
 +
 +
Dates are in the format dd.mm.
See also [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs page].
See also [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs page].
 +
 +
==Stored samples==
===Resuspended DNA===
===Resuspended DNA===
Line 9: Line 13:
! Name/abbreviation ||  Date stored ||  Description  || Source|| Backbone
! Name/abbreviation ||  Date stored ||  Description  || Source|| Backbone
|-
|-
-
|LR||? ||<partinfo>BBa_C0062</partinfo>||(RS)||pSB1A2
+
|LR||? ||<partinfo>BBa_C0062</partinfo>||(RS)||<partinfo>pSB1A2</partinfo>
|-
|-
-
|Lysis||22.06 ||BBa_K112808||(RS)||pSB1A2
+
|Lysis||22.06 ||BBa_K112808||(RS)||<partinfo>pSB1A2</partinfo>
|-
|-
-
|C78||?||<partinfo>BBa_C0078</partinfo>||(RS)||
+
|C78||?||<partinfo>BBa_C0078</partinfo>||(RS)||<partinfo>pSB2K3</partinfo>
|-
|-
-
|C79||?||<partinfo>BBa_C0078</partinfo>||(RS)||
+
|C79||?||<partinfo>BBa_C0079</partinfo>||(RS)||<partinfo>pSB2K3</partinfo>
|-
|-
-
|RBS||24.06||<partinfo>BBa_B0034</partinfo>||(RS)||pSB1A2
+
|RBS||24.06||<partinfo>BBa_B0034</partinfo>||(RS)||<partinfo>pSB1A2</partinfo>
|-
|-
-
|LuxI||?||<partinfo>BBa_C0061</partinfo>||(RS)||
+
|LuxI||?||<partinfo>BBa_C0061</partinfo>||(RS)||<partinfo>pSB1A2</partinfo>
|-
|-
-
|K+RBS||?||<partinfo>BBa_K081005</partinfo>||(RS)||pSB1A2
+
|K+RBS||?||<partinfo>BBa_K081005</partinfo>||(RS)||<partinfo>pSB1A2</partinfo>
|}
|}
 +
===Isolated DNA===
===Isolated DNA===
-
(Miniprep, gel extraction and PCR products)
+
(Miniprep, gel extraction and PCR products)<br>
 +
NOT included here: Digestion and ligation mixtures/products
{| class="wikitable"
{| class="wikitable"
! Name/abbreviation ||  Date stored ||  Description  || Concentration|| Backbone
! Name/abbreviation ||  Date stored ||  Description  || Concentration|| Backbone
|-
|-
-
|LuxR+Term||16.7|| || ||
+
|pTet GFP||26.07||<partinfo>BBa_I13522</partinfo>|| ||<partinfo>pSB1A2</partinfo>
|-
|-
-
|LuxI+Term||16.7|| || ||
+
|VGB+RBS+YFP+DTT||26.07|| || ||
|-
|-
-
|C79||27.06||<partinfo>BBa_C0079</partinfo>||70,8||pSB2K3
+
|VHB+RBS+YFP+DTT||26.07|| || ||
|-
|-
-
|C78||27.06||<partinfo>BBa_C0078</partinfo>||63,4||pSB2K3
+
|EYFP (RBS+ LVA- TERM)||26.07||<partinfo>BBa_E0430</partinfo> || ||pSB1A2
|-
|-
-
|RBS||27.06||<partinfo>BBa_B0034</partinfo>||30,2||pSB1A2
+
|LuxR+Term||16.7||<partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> ||37,0 ||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|GFP+Term||11.07|| || ||
+
|LuxI+Term||16.7||<partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo>||37,6 ||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|CFP+Term||11.07|| || ||
+
|LI (BBa_K092400)||?||<partinfo>BBa_K092400</partinfo>|| ||<partinfo>pSB1A2</partinfo>
|-
|-
-
|LI||?||<partinfo>BBa_K092400</partinfo>||
+
|GFP+DTT||13.07||<partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo>||41,1||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|YFP+Term||11.07||?|YFP+term (religated)||?||
+
|YFP+DTT||13.07||<partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo>||55,5||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|DLD + RBS||9.07|| || 24,1||
+
|RFP+DTT||13.07||<partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo>||40,9||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|VHB + RBS||9.07|| || 48,3||
+
|CFP+DTT||13.07||<partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo>||47,7||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|Jen1 + RBS||9.07|| || 35,3||
+
|K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8||<partinfo>pSB1A2</partinfo>
|-
|-
-
|VGB + RBS||9.07|| || 40,0||
+
|DLD + RBS||09.07||<partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> ||24,1||<partinfo>pSB1A2</partinfo>
|-
|-
-
|LR||?||<partinfo>BBa_B0062</partinfo> ||20,3||
+
|VHB + RBS||09.07||<partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> || 48,3||<partinfo>pSB1A2</partinfo>
|-
|-
-
|VGB promoter||27.06||<partinfo>BBa_K561001</partinfo>||22,9||pSB1C3
+
|Jen1 + RBS||09.07||<partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> || 35,3||<partinfo>pSB1A2</partinfo>
|-
|-
-
|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||pSB1C3
+
|VGB + RBS||09.07||<partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> || 40,0||<partinfo>pSB1A2</partinfo>
|-
|-
-
|RFP +Term||11.07|| ||
+
|pBAD+lysis||09.07|| ||23,6||<partinfo>pSB1A3</partinfo>
|-
|-
-
|Constitutive promoter||22.06|| ||27,7||
+
|RBS||06.07||<partinfo>BBa_B0034</partinfo>||44,5||<partinfo>pSB1A2</partinfo>
|-
|-
-
|DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8||
+
|LuxI||06.07||<partinfo>BBa_C0061</partinfo> ||38,6||<partinfo>pSB1A2</partinfo>
|-
|-
-
|K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8||
+
|Colicin||04.07||<partinfo>BBa_K150009</partinfo>||58,6||<partinfo>pSB1A2</partinfo>
|-
|-
-
|LuxR+HSL||24.06|| || ||
+
|VHB||04.07||<partinfo>BBa_K258005</partinfo>||44,9||<partinfo>pSB1A2</partinfo>
|-
|-
-
|pBAD+lysis||9.7|| ||23,6||
+
|Jen1||04.07||<partinfo>BBa_K284002</partinfo>||102,1||<partinfo>BBa_J63010</partinfo>
|-
|-
-
|DNA lysis||24.06|| || ||
+
|DLD||04.07||<partinfo>BBa_K284003</partinfo>||63,8||<partinfo>BBa_J63010</partinfo>
|-
|-
-
|D2||22.6||BBa_K145150||23,1||pSB1A2
+
|pBAD B1||01.07||<partinfo>BBa_K206000</partinfo>||24,0||<partinfo>pSB1A3</partinfo>
|-
|-
-
|RBS||6.07||<partinfo>BBa_B0034</partinfo>||44,5||pSB1A2
+
|pBAD B2||01.07||<partinfo>Bba_K206000</partinfo>||19||<partinfo>pSB1A3</partinfo>
|-
|-
-
|LuxI||6.07|| ||38,6||
+
|LR||?||<partinfo>BBa_C0062</partinfo> ||20,3||<partinfo>pSB1A2</partinfo>
|-
|-
-
|VHB||4.07||<partinfo>BBa_K258005</partinfo>||44,9||pSB1A2
+
|VGB promoter||27.06||<partinfo>BBa_K561001</partinfo>||22,9||<partinfo>pSB1C3</partinfo>
|-
|-
-
|Jen||4.07||<partinfo>BBa_K284002</partinfo>||102,1||
+
|C79||27.06||<partinfo>BBa_C0079</partinfo>||70,8||<partinfo>pSB2K3</partinfo>
|-
|-
-
|luxR+HSL||25.06|| ||13,3||
+
|C78||27.06||<partinfo>BBa_C0078</partinfo>||63,4||<partinfo>pSB2K3</partinfo>
|-
|-
-
|Lysis1 DNA||24.06|| || ||
+
|RBS||27.06||<partinfo>BBa_B0034</partinfo>||30,2||<partinfo>pSB1A2</partinfo>
|-
|-
-
|GFP+DTT||13.07|| ||41,1||
+
|RBS||26.06||<partinfo>BBa_B0034</partinfo>||16,8||<partinfo>pSB1A2</partinfo>
|-
|-
-
|YFP+DTT||13.07|| ||55,5||
+
|DTT Amp||26.06||<partinfo>BBa_B0015</partinfo>||35,8||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|RFP+DTT||13.07|| ||40,9||
+
|DTT Kan||26.06||<partinfo>BBa_B0015</partinfo>||26,5||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|CFP+DTT||13.07|| ||47,7||
+
|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||<partinfo>pSB1C3</partinfo>
|-
|-
-
|pBAD B1||1.07||<partinfo>Bba_K206000</partinfo>||24,0||
+
|luxR+HSL||25.06||<partinfo>BBa_R0062</partinfo> ||13,3||<partinfo>pSB1A2</partinfo>
|-
|-
-
|D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||pSB1A2
+
|Lysis1 DNA||24.06||<partinfo>BBa_K112808</partinfo> || ||<partinfo>pSB1A2</partinfo>
|-
|-
-
|pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||pSB1A3
+
|LuxR+HSL||24.06||<partinfo>BBa_R0062</partinfo> || ||<partinfo>pSB1A2</partinfo>
|-
|-
-
|Colicin||4.07||<partinfo>BBa_K150009</partinfo>||58,6||
+
|DNA lysis||24.06||<partinfo>BBa_K112808</partinfo> || ||<partinfo>pSB1A2</partinfo>
|-
|-
-
|VGB+RBS+YFP+DTT||26.07|| || ||
+
|Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||<partinfo>pSB1A2</partinfo>
|-
|-
-
|VHB+RBS+YFP+DTT||26.07|| || ||
+
|D2||22.6||<partinfo>BBa_K145150</partinfo>||23,1||<partinfo>pSB1A2</partinfo>
|-
|-
-
|EYFP+RBS+YFP+DTT||26.07|| || ||
+
|D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||<partinfo>pSB1A2</partinfo>
|-
|-
-
|pTet GFP||26.07||<partinfo>BBa_I13522</partinfo>|| ||pSB1A2
+
|RFP K||22.06||<partinfo>BBa_E1010</partinfo> || ||<partinfo>pSB2K3</partinfo>
|-
|-
-
|RBS||26.06||<partinfo>BBa_B0034</partinfo>||16,8||pSB1A2
+
|GFP AK||22.06||<partinfo>BBa_K082003</partinfo> || ||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|DTT Amp||26.06||<partinfo>BBa_B0015</partinfo>||35,8||pSB1AK3
+
|CFP A||22.06||<partinfo>BBa_E0020</partinfo>|| ||<partinfo>pSB1A2</partinfo>
|-
|-
-
|DTT Kan||26.06||<partinfo>BBa_B0015</partinfo>||26,5||pSB1AK3
+
|YFP AK||22.06 ||<partinfo>BBa_E0030</partinfo>|| ||<partinfo>pSB1AK3</partinfo>
 +
|}
 +
 
 +
===Restriction digests and ligation mixtures===
 +
(Inserts and backbones, DNA treated with enzymes)
 +
 
 +
 
 +
 
 +
{| class="wikitable"
 +
! Name/abbreviation ||Date stored|| Description ||Enzymes||
|-
|-
-
|RFP K||22.06|| || ||
+
|YFP+Term (religation)||11.07||?|YFP+term (religated)||?||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|GFP AK||22.06|| || ||
+
|GFP+Term (religation)||11.07||<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo>|| ||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|CFP A||22.06|| || ||
+
|CFP+Term (religation)||11.07||<partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> || ||<partinfo>pSB1AK3</partinfo>
|-
|-
-
|YFP AK||22.06 || ||
+
|RFP +Term (religation)||11.07||<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> || ||<partinfo>pSB1AK3</partinfo>
|}
|}
Line 134: Line 149:
! Name/abbreviation ||  Date stored ||  Strain  || Plasmid || Position  
! Name/abbreviation ||  Date stored ||  Strain  || Plasmid || Position  
|-
|-
-
|pTet mRFP (BBa_I13521)||27.07||DH5a||pSB1A2
+
|K+RBS+RFP+DTT||?||DH5a||?||C1
|-
|-
-
|pTet GFP (BBa_I13522)||27.07||DH5a||pSB1A2
+
|K+RBS+GFP+DTT||?||DH5a||?||C2
|-
|-
-
| EYFP + LVA (BBa_E0430)||27.07||DH5a||pSB1A2 ||
+
|RBS (<partinfo>BBa_B0034</partinfo>)||?||DH5a||?||C3
|-
|-
-
|RFP (<partinfo>BBa_E1010</partinfo>) ||18.07|| DH5a ||pSB2K3  ||
+
|pTet mRFP (<partinfo>BBa_I13521</partinfo>)||27.07||DH5a||<partinfo>pSB1A2</partinfo>C6
|-
|-
-
|VGB (<partinfo>BBa_K561001</partinfo>)||18.07||DH5a||pSB1C3 ||
+
|pTet GFP (<partinfo>BBa_I13522</partinfo>)||27.07||DH5a||<partinfo>pSB1A2</partinfo>C5
|-
|-
-
|LuxR+HSL (<partinfo>BBa_R0062</partinfo>)||16.07|| DH5a||pSB1A2 ||
+
| EYFP (RBS+ LVA- TERM) (<partinfo>BBa_E0430</partinfo>)||27.07||DH5a||<partinfo>pSB1A2</partinfo>||C4
|-
|-
-
|Lysis (<partinfo>BBa_K112808</partinfo>)||16.07|| DH5a||pSB1A2 ||
+
|RFP (<partinfo>BBa_E1010</partinfo>) ||18.07|| DH5a ||<partinfo>pSB2K3</partinfo> ||B6
|-
|-
-
|pBAD (<partinfo>Bba_K206000</partinfo>)||16.07|| DH5a||pSB1A3 ||
+
|VGB (<partinfo>BBa_K561001</partinfo>)||18.07||DH5a||<partinfo>pSB1C3</partinfo>||B5
|-
|-
-
|LuxR (<partinfo>BBa_C0062</partinfo>)||16.07|| DH5a||pSB1A2 ||
+
|LuxR+HSL (<partinfo>BBa_R0062</partinfo>)||16.07|| DH5a||<partinfo>pSB1A2</partinfo>||A1
|-
|-
-
|GFP (<partinfo>BBa_K082003</partinfo>)||16.07|| DH5a||pSB1AK3 ||
+
|Lysis (<partinfo>BBa_K112808</partinfo>)||16.07|| DH5a||<partinfo>pSB1A2</partinfo>||A3
|-
|-
-
|pBAD+Lysis||16.07|| DH5a||pSB1A3 ||
+
|pBAD (<partinfo>Bba_K206000</partinfo>)||16.07|| DH5a||<partinfo>pSB1A3</partinfo>||A4
|-
|-
-
|DTT (<partinfo>BBa_B0015</partinfo>||17.07|| DH5a||pSB1AK3 ||
+
|LuxR (<partinfo>BBa_C0062</partinfo>)||16.07|| DH5a||<partinfo>pSB1A2</partinfo>||A2
|-
|-
-
|D2 (<partinfo>BBa_K145150</partinfo>)||17.07|| DH5a||pSB1A2 ||
+
|GFP (<partinfo>BBa_K082003</partinfo>)||16.07|| DH5a||<partinfo>pSB1AK3</partinfo>||A5
|-
|-
-
|CFP (<partinfo>BBa_E0020</partinfo>)||17.07|| DH5a||pSB1A2 ||
+
|pBAD+Lysis||16.07|| DH5a||<partinfo>pSB1A3</partinfo> ||A6
|-
|-
-
|YFP (<partinfo>BBa_E0030</partinfo>)||17.07|| DH5a||pSB1AK3 ||
+
|DTT (<partinfo>BBa_B0015</partinfo>||17.07|| DH5a||<partinfo>pSB1AK3</partinfo> ||B1
 +
|-
 +
|D2 (<partinfo>BBa_K145150</partinfo>)||17.07|| DH5a||<partinfo>pSB1A2</partinfo> ||B4
 +
|-
 +
|CFP (<partinfo>BBa_E0020</partinfo>)||17.07|| DH5a||<partinfo>pSB1A2</partinfo> ||B2
 +
|-
 +
|YFP (<partinfo>BBa_E0030</partinfo>)||17.07|| DH5a||<partinfo>pSB1AK3</partinfo>||B3
|-
|-
|}
|}
 +
 +
===PCR Products===
 +
 +
{| class="wikitable"
 +
! Name || Target  ||  Primers || Template ||Status
 +
|-
 +
|pllD||pllD promoter|| ||K-12||Gel OK. Ligated and transformed, but unconfirmed.
 +
|-
 +
|Colicin||Colicin operon|| ||?||Gel good - strong band.
 +
|}
 +
 +
 +
===Agar plates===
 +
 +
{| class="wikitable"
 +
! Name/abbreviation ||  Date made||  Description  || Antibiotic||Plasmid
 +
|-
 +
|EYFP+RBS+Term||24.07||<partinfo>BBa_E0430</partinfo>||Amp||
 +
|-
 +
|A. vic. GFP||25.07||<partinfo>BBa_E0040</partinfo>||Amp||
 +
|-
 +
|Kolisin||2.07||<partinfo>BBa_K150009</partinfo>||Amp||
 +
|-
 +
|pTet GFP||24.07||<partinfo>BBa_I13522</partinfo>||Amp||
 +
|-
 +
|pTet mRFP||22.07||<partinfo>BBa_I13521</partinfo>||Amp||
 +
|-
 +
|psB1C3 religering||27.07|| ||Cm||pSB1C3
 +
|-
 +
|pllD in pSB1C3||27.07|| ||Cm||pSB1C3
 +
|-
 +
|BBa_E1010||17.07|| ||Kan||
 +
|-
 +
|VGB||17.07||BBa_K561001||Cm||
 +
|-
 +
|CFP||16.07|| ||Amp||
 +
|-
 +
|DTT||16.07||BBa_B0015||Kan||
 +
|-
 +
|YFP||16.07|| ||Amp||
 +
|-
 +
|VHB+RBS+YFP+DTT||24.07||VHB test construct||Amp||
 +
|-
 +
|VGB+RBS+YFP+DTT||24.07||VGB test construct||Amp||
 +
|-
 +
|RBS backbone||03.07|| ? ||Amp||
 +
|-
 +
|GFP + term (DTT)||11.7|| ||Amp||
 +
|-
 +
|Jen1||02.07||<partinfo>BBa_K284002</partinfo>||Amp||
 +
|-
 +
|BBa_B0034||22.7||RBS||Amp||
 +
|-
 +
|K+RBS+GFP+DTT||18.07|| ||Amp||
 +
|-
 +
|VHB||02.07||<partinfo>BBa_K258005</partinfo>||Amp||
 +
|-
 +
|LuxR||15.07||<partinfo>BBa_C0061</partinfo>||Amp||
 +
|-
 +
|pBAD + lysis||15.07||<partinfo>Bba_K206000</partinfo>+<partinfo>BBa_K112808</partinfo>||Amp||
 +
|-
 +
|Lysis||15.07||<partinfo>BBa_K112808</partinfo>||Amp||
 +
|-
 +
|GFP||15.07||?||Amp||
 +
|-
 +
|dld||02.07||<partinfo>BBa_K284002</partinfo>||Amp||
 +
|-
 +
|RBS Backbone + VGB||03.07|| ||Amp||
 +
|-
 +
|YFP + DTT||11.07|| ||Amp||
 +
|-
 +
|GFP + DTT||11.07|| ||Amp||
 +
|-
 +
|RFP + DTT||11.07|| ||Amp||
 +
|-
 +
|K+RBS+YFP+DTT||17.07|| ||Amp||
 +
|-
 +
|VGB+RBS+GFP+DTT||17.07|| ||Amp||
 +
|-
 +
|VGB+RBS+RFP+DTT||17.07|| ||Amp||
 +
|-
 +
|pBAD||16.07|| ||Amp||
 +
|-
 +
|Jen1+RBS+GFP+DTT||17.07|| ||Amp||
 +
|-
 +
|LuxR+HSL||15.07|| ||Amp||
 +
|-
 +
|VHB + RBS||06.07|| ||Amp||
 +
|-
 +
|VGB+RBS||06.07|| ||Amp||
 +
|-
 +
|dld+RBS||06.07|| ||Amp||
 +
|-
 +
|RBS backbone + VGB||03.07||Amp||
 +
|-
 +
|Jen1+RBS||06.07|| ||Amp||
 +
|-
 +
|LuxR+DTT||12.07||<partinfo>BBa_C0062</partinfo>+<partinfo>BBa_B0015</partinfo> ||Amp||
 +
|-
 +
|LuxI+DTT||12.07|| ||Amp||
 +
|-
 +
|K+RBS||11.07||<partinfo>BBa_K081005</partinfo>||Amp||
 +
|}
 +
 +
 +
Agar plate log:

Latest revision as of 19:50, 29 July 2012

This page lists stored cultures and other samples:

Dates are in the format dd.mm.

See also Constructs page.

Contents

Stored samples

Resuspended DNA

(Dried DNA from distribution kit resuspended in dH2O)

Name/abbreviation Date stored Description Source Backbone
LR? <partinfo>BBa_C0062</partinfo>(RS)<partinfo>pSB1A2</partinfo>
Lysis22.06 BBa_K112808(RS)<partinfo>pSB1A2</partinfo>
C78?<partinfo>BBa_C0078</partinfo>(RS)<partinfo>pSB2K3</partinfo>
C79?<partinfo>BBa_C0079</partinfo>(RS)<partinfo>pSB2K3</partinfo>
RBS24.06<partinfo>BBa_B0034</partinfo>(RS)<partinfo>pSB1A2</partinfo>
LuxI?<partinfo>BBa_C0061</partinfo>(RS)<partinfo>pSB1A2</partinfo>
K+RBS?<partinfo>BBa_K081005</partinfo>(RS)<partinfo>pSB1A2</partinfo>

Isolated DNA

(Miniprep, gel extraction and PCR products)
NOT included here: Digestion and ligation mixtures/products

Name/abbreviation Date stored Description Concentration Backbone
pTet GFP26.07<partinfo>BBa_I13522</partinfo> <partinfo>pSB1A2</partinfo>
VGB+RBS+YFP+DTT26.07
VHB+RBS+YFP+DTT26.07
EYFP (RBS+ LVA- TERM)26.07<partinfo>BBa_E0430</partinfo> pSB1A2
LuxR+Term16.7<partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> 37,0 <partinfo>pSB1AK3</partinfo>
LuxI+Term16.7<partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo>37,6 <partinfo>pSB1AK3</partinfo>
LI (BBa_K092400)?<partinfo>BBa_K092400</partinfo> <partinfo>pSB1A2</partinfo>
GFP+DTT13.07<partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo>41,1<partinfo>pSB1AK3</partinfo>
YFP+DTT13.07<partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo>55,5<partinfo>pSB1AK3</partinfo>
RFP+DTT13.07<partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo>40,9<partinfo>pSB1AK3</partinfo>
CFP+DTT13.07<partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo>47,7<partinfo>pSB1AK3</partinfo>
K+RBS13.07<partinfo>BBa_K081005</partinfo>19,8<partinfo>pSB1A2</partinfo>
DLD + RBS09.07<partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> 24,1<partinfo>pSB1A2</partinfo>
VHB + RBS09.07<partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> 48,3<partinfo>pSB1A2</partinfo>
Jen1 + RBS09.07<partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> 35,3<partinfo>pSB1A2</partinfo>
VGB + RBS09.07<partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> 40,0<partinfo>pSB1A2</partinfo>
pBAD+lysis09.07 23,6<partinfo>pSB1A3</partinfo>
RBS06.07<partinfo>BBa_B0034</partinfo>44,5<partinfo>pSB1A2</partinfo>
LuxI06.07<partinfo>BBa_C0061</partinfo> 38,6<partinfo>pSB1A2</partinfo>
Colicin04.07<partinfo>BBa_K150009</partinfo>58,6<partinfo>pSB1A2</partinfo>
VHB04.07<partinfo>BBa_K258005</partinfo>44,9<partinfo>pSB1A2</partinfo>
Jen104.07<partinfo>BBa_K284002</partinfo>102,1<partinfo>BBa_J63010</partinfo>
DLD04.07<partinfo>BBa_K284003</partinfo>63,8<partinfo>BBa_J63010</partinfo>
pBAD B101.07<partinfo>BBa_K206000</partinfo>24,0<partinfo>pSB1A3</partinfo>
pBAD B201.07<partinfo>Bba_K206000</partinfo>19<partinfo>pSB1A3</partinfo>
LR?<partinfo>BBa_C0062</partinfo> 20,3<partinfo>pSB1A2</partinfo>
VGB promoter27.06<partinfo>BBa_K561001</partinfo>22,9<partinfo>pSB1C3</partinfo>
C7927.06<partinfo>BBa_C0079</partinfo>70,8<partinfo>pSB2K3</partinfo>
C7827.06<partinfo>BBa_C0078</partinfo>63,4<partinfo>pSB2K3</partinfo>
RBS27.06<partinfo>BBa_B0034</partinfo>30,2<partinfo>pSB1A2</partinfo>
RBS26.06<partinfo>BBa_B0034</partinfo>16,8<partinfo>pSB1A2</partinfo>
DTT Amp26.06<partinfo>BBa_B0015</partinfo>35,8<partinfo>pSB1AK3</partinfo>
DTT Kan26.06<partinfo>BBa_B0015</partinfo>26,5<partinfo>pSB1AK3</partinfo>
VGB DNA25.06<partinfo>BBa_K561001</partinfo> <partinfo>pSB1C3</partinfo>
luxR+HSL25.06<partinfo>BBa_R0062</partinfo> 13,3<partinfo>pSB1A2</partinfo>
Lysis1 DNA24.06<partinfo>BBa_K112808</partinfo> <partinfo>pSB1A2</partinfo>
LuxR+HSL24.06<partinfo>BBa_R0062</partinfo> <partinfo>pSB1A2</partinfo>
DNA lysis24.06<partinfo>BBa_K112808</partinfo> <partinfo>pSB1A2</partinfo>
Constitutive promoter22.06<partinfo>BBa_J23119</partinfo> 27,7<partinfo>pSB1A2</partinfo>
D222.6<partinfo>BBa_K145150</partinfo>23,1<partinfo>pSB1A2</partinfo>
D122.06<partinfo>BBa_K091146</partinfo>19,8<partinfo>pSB1A2</partinfo>
RFP K22.06<partinfo>BBa_E1010</partinfo> <partinfo>pSB2K3</partinfo>
GFP AK22.06<partinfo>BBa_K082003</partinfo> <partinfo>pSB1AK3</partinfo>
CFP A22.06<partinfo>BBa_E0020</partinfo> <partinfo>pSB1A2</partinfo>
YFP AK22.06 <partinfo>BBa_E0030</partinfo> <partinfo>pSB1AK3</partinfo>

Restriction digests and ligation mixtures

(Inserts and backbones, DNA treated with enzymes)


Name/abbreviation Date stored Description Enzymes
YFP+Term (religation)11.07YFP+term (religated)?<partinfo>pSB1AK3</partinfo>
GFP+Term (religation)11.07<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>
CFP+Term (religation)11.07<partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>
RFP +Term (religation)11.07<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>

Glycerol stocks

(Cell cultures kept at -80 C)

Name/abbreviation Date stored Strain Plasmid Position
K+RBS+RFP+DTT?DH5a?C1
K+RBS+GFP+DTT?DH5a?C2
RBS (<partinfo>BBa_B0034</partinfo>)?DH5a?C3
pTet mRFP (<partinfo>BBa_I13521</partinfo>)27.07DH5a<partinfo>pSB1A2</partinfo>C6
pTet GFP (<partinfo>BBa_I13522</partinfo>)27.07DH5a<partinfo>pSB1A2</partinfo>C5
EYFP (RBS+ LVA- TERM) (<partinfo>BBa_E0430</partinfo>)27.07DH5a<partinfo>pSB1A2</partinfo>C4
RFP (<partinfo>BBa_E1010</partinfo>) 18.07 DH5a <partinfo>pSB2K3</partinfo> B6
VGB (<partinfo>BBa_K561001</partinfo>)18.07DH5a<partinfo>pSB1C3</partinfo>B5
LuxR+HSL (<partinfo>BBa_R0062</partinfo>)16.07 DH5a<partinfo>pSB1A2</partinfo>A1
Lysis (<partinfo>BBa_K112808</partinfo>)16.07 DH5a<partinfo>pSB1A2</partinfo>A3
pBAD (<partinfo>Bba_K206000</partinfo>)16.07 DH5a<partinfo>pSB1A3</partinfo>A4
LuxR (<partinfo>BBa_C0062</partinfo>)16.07 DH5a<partinfo>pSB1A2</partinfo>A2
GFP (<partinfo>BBa_K082003</partinfo>)16.07 DH5a<partinfo>pSB1AK3</partinfo>A5
pBAD+Lysis16.07 DH5a<partinfo>pSB1A3</partinfo> A6
DTT (<partinfo>BBa_B0015</partinfo>17.07 DH5a<partinfo>pSB1AK3</partinfo> B1
D2 (<partinfo>BBa_K145150</partinfo>)17.07 DH5a<partinfo>pSB1A2</partinfo> B4
CFP (<partinfo>BBa_E0020</partinfo>)17.07 DH5a<partinfo>pSB1A2</partinfo> B2
YFP (<partinfo>BBa_E0030</partinfo>)17.07 DH5a<partinfo>pSB1AK3</partinfo>B3

PCR Products

Name Target Primers Template Status
pllDpllD promoter K-12Gel OK. Ligated and transformed, but unconfirmed.
ColicinColicin operon ?Gel good - strong band.


Agar plates

Name/abbreviation Date made Description AntibioticPlasmid
EYFP+RBS+Term24.07<partinfo>BBa_E0430</partinfo>Amp
A. vic. GFP25.07<partinfo>BBa_E0040</partinfo>Amp
Kolisin2.07<partinfo>BBa_K150009</partinfo>Amp
pTet GFP24.07<partinfo>BBa_I13522</partinfo>Amp
pTet mRFP22.07<partinfo>BBa_I13521</partinfo>Amp
psB1C3 religering27.07 CmpSB1C3
pllD in pSB1C327.07 CmpSB1C3
BBa_E101017.07 Kan
VGB17.07BBa_K561001Cm
CFP16.07 Amp
DTT16.07BBa_B0015Kan
YFP16.07 Amp
VHB+RBS+YFP+DTT24.07VHB test constructAmp
VGB+RBS+YFP+DTT24.07VGB test constructAmp
RBS backbone03.07 ? Amp
GFP + term (DTT)11.7 Amp
Jen102.07<partinfo>BBa_K284002</partinfo>Amp
BBa_B003422.7RBSAmp
K+RBS+GFP+DTT18.07 Amp
VHB02.07<partinfo>BBa_K258005</partinfo>Amp
LuxR15.07<partinfo>BBa_C0061</partinfo>Amp
pBAD + lysis15.07<partinfo>Bba_K206000</partinfo>+<partinfo>BBa_K112808</partinfo>Amp
Lysis15.07<partinfo>BBa_K112808</partinfo>Amp
GFP15.07?Amp
dld02.07<partinfo>BBa_K284002</partinfo>Amp
RBS Backbone + VGB03.07 Amp
YFP + DTT11.07 Amp
GFP + DTT11.07 Amp
RFP + DTT11.07 Amp
K+RBS+YFP+DTT17.07 Amp
VGB+RBS+GFP+DTT17.07 Amp
VGB+RBS+RFP+DTT17.07 Amp
pBAD16.07 Amp
Jen1+RBS+GFP+DTT17.07 Amp
LuxR+HSL15.07 Amp
VHB + RBS06.07 Amp
VGB+RBS06.07 Amp
dld+RBS06.07 Amp
RBS backbone + VGB03.07Amp
Jen1+RBS06.07 Amp
LuxR+DTT12.07<partinfo>BBa_C0062</partinfo>+<partinfo>BBa_B0015</partinfo> Amp
LuxI+DTT12.07 Amp
K+RBS11.07<partinfo>BBa_K081005</partinfo>Amp


Agar plate log:

Retrieved from "http://2012.igem.org/Team:NTNU_Trondheim/Stocks"