Team:NTNU Trondheim/Constructs

From 2012.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:NTNU_Trondheim/Templates/Header}}
{{:Team:NTNU_Trondheim/Templates/Header}}
</div>
</div>
 +
 +
{| class="wikitable" id="planTable"
 +
|+ Test and working constructs
 +
! Navn ||  BioBricks (5'-3') ||  Funksjon || Backbone (length)  || Copy number ||Status
 +
|-
 +
|pllD|| || pllD promoter in pSB1C3||<partinfo>pSB1C3</partinfo>||High||Transformed but unverified
 +
|-
 +
|VGB+RBS+YFP+DTT||<partinfo>BBa_K561001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0030</partinfo>,<partinfo>BBa_B0015</partinfo>||VGB test construct||?||?||Transformed. Untested.
 +
|-
 +
|VHB+RBS+YFP+DTT||<partinfo>BBa_K258005</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>||VHB test construct||?||?||Transformed. Untested.
 +
|-
 +
|K+RBS+RFP+DTT||<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_E1010</partinfo>,<partinfo>BBa_B0015</partinfo>||RFP test construct||?||?||Negative results
 +
|-
 +
|K+RBS+YFP+DTT||<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_E0030</partinfo>,<partinfo>BBa_B0015</partinfo>||YFP test construct||?||?||Works
 +
|-
 +
|K+RBS+GFP+DTT||<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_K082003</partinfo>,<partinfo>BBa_B0015</partinfo>||GFP test construct||?||?||Negative results
 +
|-
 +
|PBAD+lysis|| <partinfo>BBa_K206000</partinfo>, <partinfo>BBa_K112808</partinfo>||Arabinoseindusert promotor med lysis device|| <partinfo>pSB1A3</partinfo> || ||Testet og fungerer
 +
|}
{| class="wikitable"
{| class="wikitable"
 +
|+ Intermediates (constructs that have no function alone)
! Navn ||  BioBricks (5'-3') ||  Funksjon || Backbone (length)  || Copy number ||Status  
! Navn ||  BioBricks (5'-3') ||  Funksjon || Backbone (length)  || Copy number ||Status  
|-
|-
-
|DLD+RBS|| BBa_K284003, BBa_B0034|| Laktatpromotor med RBS|| pSB1A2 (2079)|| High ||Testkuttet
+
|DLD+RBS|| <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>||Laktatpromotor med RBS||<partinfo>pSB1A2</partinfo> (2079)|| High ||Testkuttet
-
|-
+
-
|JEN1+RBS|| BBa_K284003, BBa_B0034|| Laktatpromotor med RBS|| pSB1A2 (2079) || High ||Testkuttet
+
|-
|-
-
|VGB+RBS ||BBa_K284003, BBa_B0034|| Oksygenpromotor med RBS || pSB1A2 (2079) || High ||Testkuttet
+
|JEN1+RBS|| <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>||Laktatpromotor med RBS||<partinfo>pSB1A2</partinfo>  (2079) || High ||Testkuttet
|-
|-
-
|VHB+RBS|| BBa_K284003, BBa_B0034|| Oksygenpromotor med RBS || pSB1A2 (2079) || High ||Testkuttet
+
|VGB+RBS ||<partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>||Oksygenpromotor med RBS ||<partinfo>pSB1A2</partinfo> (2079) || High ||Testkuttet
|-
|-
-
|RFP+DTT ||Bba_E1010, BBa_B0015|| Fluorescerende protein med terminator|| ? || ||Testkuttet
+
|VHB+RBS|| <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>||Oksygenpromotor med RBS ||<partinfo>pSB1A2</partinfo>  (2079) || High ||Testkuttet
|-
|-
-
|YFP+DTT ||BBa_E0030, BBa_B0015|| Fluorescerende protein med terminator|| pSB1AK3 (3189)|| ||Testkuttet
+
|RFP+DTT ||<partinfo>Bba_E1010</partinfo>, <partinfo>BBa_B0015</partinfo>||Fluorescerende protein med terminator||<partinfo>pSB1AK3</partinfo> (3189) || ||Testkuttet
|-
|-
-
|CFP+DTT ||BBa_E0020, BBa_B0015|| Fluorescerende protein med terminator|| ? || ||Testkuttet
+
|YFP+DTT ||<partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>||Fluorescerende protein med terminator||<partinfo>pSB1AK3</partinfo>  (3189)|| ||Testkuttet
|-
|-
-
|GFP(+LVA)+DTT ||BBa_K082003, BBa_B0015 ||Fluorescerende protein med terminator|| ? || ||Testkuttet
+
|CFP+DTT ||<partinfo>BBa_E0020</partinfo>, <partinfo>BBa_B0015</partinfo>||Fluorescerende protein med terminator||<partinfo>pSB1AK3</partinfo>  (3189) || ||Testkuttet
|-
|-
-
|PBAD+lysis|| BBa_K206000, BBa_K112808||Arabinoseindusert promotor med lysis device|| pSB1A3 || ||Testet og fungerer
+
|GFP(+LVA)+DTT ||<partinfo>BBa_K082003</partinfo>, <partinfo>BBa_B0015</partinfo>||Fluorescerende protein med terminator||<partinfo>pSB1AK3</partinfo>  || ||Testkuttet
|-
|-
-
|LuxR+DTT|| BBa_C0062, BBa_B0015|| LuxR-gen med terminator||? || ||Ligert og transformert
+
|LuxR+DTT|| <partinfo>BBa_C0062</partinfo>, <partinfo>BBa_B0015</partinfo>||LuxR-gen med terminator||<partinfo>pSB1AK3</partinfo> || ||Ligert og transformert
|-
|-
-
|LuxI+DTT|| BBa_C0061, BBa_B0015|| LuxI-gen med terminator||? || ||Ligert og transformert
+
|LuxI+DTT|| <partinfo>BBa_C0061</partinfo>, <partinfo>BBa_B0015</partinfo>||LuxI-gen med terminator||<partinfo>pSB1AK3</partinfo> || ||Ligert og transformert
|-
|-
|}
|}

Latest revision as of 11:38, 8 August 2012

Search and Destroy
Search and Destroy
NTNU IS B.A.C.K.
Bacterial Anti-Cancer-Kamikaze
</div>

Test and working constructs
Navn BioBricks (5'-3') Funksjon Backbone (length) Copy number Status
pllD pllD promoter in pSB1C3<partinfo>pSB1C3</partinfo>HighTransformed but unverified
VGB+RBS+YFP+DTT<partinfo>BBa_K561001</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0030</partinfo>,<partinfo>BBa_B0015</partinfo>VGB test construct??Transformed. Untested.
VHB+RBS+YFP+DTT<partinfo>BBa_K258005</partinfo>, <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>VHB test construct??Transformed. Untested.
K+RBS+RFP+DTT<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_E1010</partinfo>,<partinfo>BBa_B0015</partinfo>RFP test construct??Negative results
K+RBS+YFP+DTT<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_E0030</partinfo>,<partinfo>BBa_B0015</partinfo>YFP test construct??Works
K+RBS+GFP+DTT<partinfo>BBa_K081005</partinfo>,<partinfo>BBa_K082003</partinfo>,<partinfo>BBa_B0015</partinfo>GFP test construct??Negative results
PBAD+lysis <partinfo>BBa_K206000</partinfo>, <partinfo>BBa_K112808</partinfo>Arabinoseindusert promotor med lysis device <partinfo>pSB1A3</partinfo> Testet og fungerer
Intermediates (constructs that have no function alone)
Navn BioBricks (5'-3') Funksjon Backbone (length) Copy number Status
DLD+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Laktatpromotor med RBS<partinfo>pSB1A2</partinfo> (2079) High Testkuttet
JEN1+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Laktatpromotor med RBS<partinfo>pSB1A2</partinfo> (2079) High Testkuttet
VGB+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Oksygenpromotor med RBS <partinfo>pSB1A2</partinfo> (2079) High Testkuttet
VHB+RBS <partinfo>BBa_K284003</partinfo>, <partinfo>BBa_B0034</partinfo>Oksygenpromotor med RBS <partinfo>pSB1A2</partinfo> (2079) High Testkuttet
RFP+DTT <partinfo>Bba_E1010</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> (3189) Testkuttet
YFP+DTT <partinfo>BBa_E0030</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> (3189) Testkuttet
CFP+DTT <partinfo>BBa_E0020</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> (3189) Testkuttet
GFP(+LVA)+DTT <partinfo>BBa_K082003</partinfo>, <partinfo>BBa_B0015</partinfo>Fluorescerende protein med terminator<partinfo>pSB1AK3</partinfo> Testkuttet
LuxR+DTT <partinfo>BBa_C0062</partinfo>, <partinfo>BBa_B0015</partinfo>LuxR-gen med terminator<partinfo>pSB1AK3</partinfo> Ligert og transformert
LuxI+DTT <partinfo>BBa_C0061</partinfo>, <partinfo>BBa_B0015</partinfo>LuxI-gen med terminator<partinfo>pSB1AK3</partinfo> Ligert og transformert
Retrieved from "http://2012.igem.org/Team:NTNU_Trondheim/Constructs"