Team:Arizona State

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We are Arizona State University's first iGEM team, working over the summer for the 2011 International Genetically Engineered Machine competition.
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== Abstract ==
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<p>Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are a genomic feature of many prokaryotic and archaeal species. CRISPR functions as an adaptive immune system, targeting exogenous sequences that match spacers integrated into the genome. Our project focuses on developing a set of tools for synthetic control over the CRISPR pathway. This includes a method for creating polymers of repeat-spacer-repeat units, the development of CRISPR biobricks (CAS genes, leader sequences) for several CRISPR subtypes (E. coli, B. halodurans, and L. innocua), testing these components on plasmids containing GFP, and a software tool to collect and display CRISPR information, as well as select spacers from a particular sequence. Given the relatively recent progress in the scientific understanding of this system, we see the potential for a wide range of biotechnological applications of CRISPR in the future.</p>
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''[[Team:Arizona State/Project/Introduction|more]]''
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== What is CRISPR? ==
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<p>'''C'''lustered '''R'''egularly '''I'''nterspaced '''S'''hort '''P'''alindromic '''R'''epeats (CRISPR) are a genomic feature of many prokaryotic and archeal species. CRISPR functions as an adaptive immune system. A CRISPR locus consists of a set of CAS (CRISPR associated) genes, a leader, or promoter, sequence, and an array. This array consists of repeating elements along with "spacers". These spacer regions direct the CRISPR machinery to degrade or otherwise inactivate a complementary sequence in the cell.</p>
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[[Image:ASU Crispr basic.png|600px|Basic mechanism]]<br>
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    <td width="415"><h4>The Problem:<br />
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A basic diagram of the CRISPR pathway. In this image, a CRISPR array is transcribed and used to locate a complementary sequence in the cell for degradation. '''[[Team:Arizona State/Project/CRISPR|More information]]'''.
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      Childhood Diarrhea From Contaminated Water</h4></td>
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    <td colspan="2"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/Problem"><img src="https://static.igem.org/mediawiki/2012/0/03/Asuigem_diseasemap.png" alt="" width="450" /></td>
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    <td colspan="2"><medium>Viewed as a minor inconvenience in the developed world, diarrhea can be a death sentence in developing countries. Diarrhea can be life threatening as it causes severe dehydration as a result of extensive fluid loss. An estimated 2.0 billion cases of diarrhea occur each year amongst children under five years of age. Of these cases, 1.5 million children die. The major bacterial pathogens that most frequently cause acute childhood diarrhea are  <i>E. coli, Shigella, Campylobacter</i> and <i>Salmonella</i>. Currently, existing biosensors for water-borne pathogens are either costly, inaccessible to developing countries, require expensive equipment constrained to a lab setting, difficult to use without training, and not very reliable...<a href="https://2012.igem.org/Team:Arizona_State/Problem"><strong>More &gt;</strong></a></small></td>
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          <td width="155"><p align="center"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/Overview">
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          <td width="155"><p align="center"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/Team">
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          <td><p align="center"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/Data">
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          <td><p align="center"><a class="linkopacity" href="https://2012.igem.org/Team:Arizona_State/International">
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  <p>&nbsp;</p>
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Latest revision as of 03:39, 27 October 2012



The Problem:
Childhood Diarrhea From Contaminated Water
Viewed as a minor inconvenience in the developed world, diarrhea can be a death sentence in developing countries. Diarrhea can be life threatening as it causes severe dehydration as a result of extensive fluid loss. An estimated 2.0 billion cases of diarrhea occur each year amongst children under five years of age. Of these cases, 1.5 million children die. The major bacterial pathogens that most frequently cause acute childhood diarrhea are E. coli, Shigella, Campylobacter and Salmonella. Currently, existing biosensors for water-borne pathogens are either costly, inaccessible to developing countries, require expensive equipment constrained to a lab setting, difficult to use without training, and not very reliable...More >

 

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Arizona State University
ECG 334, PO BOX 9709
Tempe, Arizona 85287
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