Team:HIT-Harbin

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<li class="page_item page-item-136"><a href="https://2012.igem.org/Team:HIT-Harbin/team" title="OVERVIEW">OVERVIEW</a></li>
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<div class="post-title-large"><a>History of HIT</a></div>
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<div class="post-excerpt"><p>This is the oldest building of Harbin Institute of Technology</p>
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<div class="post-title-large"><a>Centre Street in Snow</a></div>
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<div class="post-title-large"><a>Project Overview</a></div>
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<div class="post-excerpt"><p>A visual description of the whole lab work we've done</p>
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<div class="post-excerpt"><p>For the birthday celebration of our former team leader</p>
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<div class="post-excerpt"><p>We have a wonderful experience in the lab this summer</p>
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<a href = "https://2012.igem.org/Team:HIT-Harbin/project/part1">Detecting Device</a>
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<p>We use engineered E. coli.to detect the existence of S.aureus in the environment through sensing the AIPs secreted only from S.aureus, and the report will be shown by the expression of GFP.</p>
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<a href = "https://2012.igem.org/Team:HIT-Harbin/project/part2">Killing Device</a>
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<p>The construct is designed to eradicate S.aureus through the production of lysostaphin, which would be released till a certain concentration.</p>
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<a href = "https://2012.igem.org/Team:HIT-Harbin/project/part3">Biofilm Formation Device</a>
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<p>In order to increase bactericidal effect, we enhance biofilm formation by over-expression of yddV, a di-guanylate cyclase, which catalyzes GTP into c-di-GMP. </p>
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<a href = "https://2012.igem.org/Team:HIT-Harbin/project/application">Application</a>
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<p>The system we have designed can be applied to the pools which contain flowing water with existence of S.aureus. For further application, this device can developed for medical use.</p>
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<a href="https://2012.igem.org/Team:HIT-Harbin/team" title="About us">About us</a>
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<p>&nbsp;&nbsp;&nbsp;&nbsp;The HIT-Harbin team is composed of 15 motivated students across a wide range of academic backgrounds this year: from freshman to senior, from biology to electromechanics. The identical dream makes us together to share a passion for science, stay-up late in the lab and learn. We're currently taking great efforts to make it and enjoying the happiness and sadness it bring to us.</p>
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<p><em>Staphylococcus aureus</em> infections are a major caus of morbidity and  mortality in community and hospital settings. Consequently, the emergence of  methicillin-resistant and, more recently, vancomysin-resistant strains of <em>S.aureus</em> represents an enormous threat to public health. Since bacterial sensors are  attracting more and more biologists' attention owing to its detecting  specifically, quickly and accurately, we plan to construct a <em>E.coli</em> consortium consisting of two different engineered populations, which comprises  consortium biofilm formation, <em>S.aureus </em>detecting, killing and bacterial lysis devices, that will enable the <em>E.coli</em> consortium to detect and kill  a pathogen <em>S.aureus</em>.<br />
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  Firstly, a detecting system will be constructed. The engineered can detect the existence of <em>S.aureus </em>through sensing  the AIPs secreted only from <em>S.aureus</em>. The report will be shown  by the expression of GFP. Once it senses the AIPs, the engineered<em> E.coli</em> will release  signal protein AHL into the environment.<br />
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  Secondly, a killing and lysis system will be constructed. Upon sensing  the AHL, the engineered <em>E.coli </em>will express lysostaphin, an endopeptidase,  which cleaves the pentaglycine cross-bridges of the staphylococal cell wall  rapidly lysing the bacteria. Some time after the lysostaphin is expressed, the  lysis E7 will be expressed and lysis <em>E.coli </em>so that  lysostaphin can be released into the environment.</p>
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<a href="http://jcxb.hit.edu.cn/">
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Finally, we will construct an enhanced consortium biofilm. The consortium biofilm is  composed of the two different engineered <em>E.coli</em> populations, which is  able to sense and kill <em>S.aureus</em>, respectively. The  enhanced consortium biofilm can promote intra- and inter-species signal  transduction, and stay stably. The detecting and killing system is distributed  in two different engineered <em>E.coli</em>. This will make functions  compartmental, and reduce metabolism load. On the other hand, this will promote  modularity in the level of bacteria
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Latest revision as of 13:59, 17 September 2013

HIT-Harbin

Detecting Device

We use engineered E. coli.to detect the existence of S.aureus in the environment through sensing the AIPs secreted only from S.aureus, and the report will be shown by the expression of GFP.

Killing Device

The construct is designed to eradicate S.aureus through the production of lysostaphin, which would be released till a certain concentration.

Biofilm Formation Device

In order to increase bactericidal effect, we enhance biofilm formation by over-expression of yddV, a di-guanylate cyclase, which catalyzes GTP into c-di-GMP.

Application

The system we have designed can be applied to the pools which contain flowing water with existence of S.aureus. For further application, this device can developed for medical use.

About us

    The HIT-Harbin team is composed of 15 motivated students across a wide range of academic backgrounds this year: from freshman to senior, from biology to electromechanics. The identical dream makes us together to share a passion for science, stay-up late in the lab and learn. We're currently taking great efforts to make it and enjoying the happiness and sadness it bring to us.

SPONSORS

Back to Top

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