Team:Columbia-Cooper-NYC/Making Liquid Media
From 2012.igem.org
(Difference between revisions)
(→Liquid Media Protocol) |
|||
(2 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
+ | <html> | ||
+ | <head> | ||
+ | <style type="text/css"> | ||
+ | body{background-image:url('http://farm9.staticflickr.com/8039/7990439399_1970f791f6_h.jpg'); | ||
+ | background-position:top left; | ||
+ | background-repeat:repeat-y | ||
+ | |||
+ | body{background-image:url('http://farm9.staticflickr.com/8173/7989724336_9e5441e3ff_b.jpg'); | ||
+ | background-position:top right; | ||
+ | background-repeat:repeat-y | ||
+ | } | ||
+ | |||
+ | |||
+ | |||
+ | </style> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | |||
<html> | <html> | ||
<head> | <head> | ||
Line 144: | Line 164: | ||
<div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> | <div id="m7" onmouseover="mcancelclosetime()" onmouseout="mclosetime()"> | ||
<a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a> | <a href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Safety">Safety</a> | ||
- | + | </div> | |
</li> | </li> | ||
<li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a> | <li><a href="#" onmouseover="mopen('m8')" onmouseout="mclosetime()">Sponsors</a> | ||
Line 175: | Line 195: | ||
<LI>1.0 g of magnesium sulfate MgSO4 | <LI>1.0 g of magnesium sulfate MgSO4 | ||
<LI>0.4 g of dipotassium phosphate K2HPO4 | <LI>0.4 g of dipotassium phosphate K2HPO4 | ||
- | <LI>5.0 mL | + | <LI>5.0 mL of MD-TMS |
</UL> | </UL> | ||
- | <LI>Using sulfuric acid ( | + | <LI>Using sulfuric acid (H2SO4), adjust pH of solution to 2.1. (This should take about 0.5 mL of H2SO4.) |
- | <LI>Add 20 g of FeSO4 | + | <LI>Add 20 g of FeSO4*7H20 |
<LI>Using sulfuric acid, adjust pH of solution to 1.8-1.85 | <LI>Using sulfuric acid, adjust pH of solution to 1.8-1.85 | ||
<LI>Once solution appears homogeneous, filter sterilize | <LI>Once solution appears homogeneous, filter sterilize | ||
Line 184: | Line 204: | ||
<i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i> | <i>Credit for protocol goes to Jason Candreva, Research Associate, Columbia University</i> | ||
+ | |||
+ | Return to [[Team:Columbia-Cooper-NYC/Protocols|Protocols Page]] |
Latest revision as of 07:47, 7 January 2013
Liquid Media Protocol
For 1 liter of liquid media:
- Combine in a large beaker, while stirring with a magnetic bar:
- 1 L of distilled water
- 0.8 g of ammonium sulfate (NH4)2SO4
- 1.0 g of magnesium sulfate MgSO4
- 0.4 g of dipotassium phosphate K2HPO4
- 5.0 mL of MD-TMS
- Using sulfuric acid (H2SO4), adjust pH of solution to 2.1. (This should take about 0.5 mL of H2SO4.)
- Add 20 g of FeSO4*7H20
- Using sulfuric acid, adjust pH of solution to 1.8-1.85
- Once solution appears homogeneous, filter sterilize
Credit for protocol goes to Jason Candreva, Research Associate, Columbia University
Return to Protocols Page