Team:UIUC-Illinois/Results
From 2012.igem.org
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<center><h2>PUF Experimental Results</h2></center> | <center><h2>PUF Experimental Results</h2></center> | ||
<li><a name="data0" >Overview</a></li> | <li><a name="data0" >Overview</a></li> | ||
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<li><a name="data2" >YFP Fluorescence Results</a></li> | <li><a name="data2" >YFP Fluorescence Results</a></li> | ||
<li><a name="data3" >mCherry Fluorescence Results</a></li> | <li><a name="data3" >mCherry Fluorescence Results</a></li> | ||
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<center><h2>YFP Fluorescence Data</h2></center><br/> | <center><h2>YFP Fluorescence Data</h2></center><br/> | ||
- | <img src="https://static.igem.org/mediawiki/2012/ | + | <img src="https://static.igem.org/mediawiki/2012/d/d2/FixedYFPfltest.png" height=400 width=715> |
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- | With regards to our hypothetical results, our experimental fluorescence measurements were substantially faithful to our predictions. Looking at columns 5, 6, 7, and 8, our observations appear to be in line with what we have predicted in introducing the wild type PUF-PIN to constructs containing | + | With regards to our hypothetical results, our experimental fluorescence measurements were substantially faithful to our predictions. Looking at columns 5, 6, 7, and 8, our observations appear to be in line with what we have predicted in introducing the wild type PUF-PIN to constructs containing specific recognition site. However, YFP expression was silenced regardless of the binding site type present in the YFP expression constructs. With many control experiments and repeated tests, we thought the most possible reason could be that PUF recognizes sequences in the YFP gene (although the YFP sequences provided from the registry showed no PUF binding sites <a href="http://partsregistry.org/cgi/partsdb/puttext.cgi">in silico</a>). Columns 1 and 2 are negative fluorescence controls while Columns 3, 4, 5, and 6 are positive fluorescence controls. |
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<center><h2>mCherry Fluorescence Data</h2></center><br/> | <center><h2>mCherry Fluorescence Data</h2></center><br/> | ||
- | <img src="https://static.igem.org/mediawiki/2012/ | + | <img src="https://static.igem.org/mediawiki/2012/f/f3/NEWmcherry.png" height=100% width=100%> |
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In order to facilitate more reliable results, we also tested our constructs with the expression of an RFP, mCherry. If in the case that confounding factors influenced our results in our experiments using YFP, we could minimize inaccurate influences on our data by using a different reporter. <br/><br/> | In order to facilitate more reliable results, we also tested our constructs with the expression of an RFP, mCherry. If in the case that confounding factors influenced our results in our experiments using YFP, we could minimize inaccurate influences on our data by using a different reporter. <br/><br/> | ||
- | + | As of now, we are still running more mcherry experiments. The data showed some preliminary results. | |
+ | The results confirmed our hypothesis that PUF-PIN don’t silence the gene from the control construct. This confirms our hypothesis that PUF-PIN doesn't silence the gene from the control construct. | ||
</div> | </div> |
Latest revision as of 21:10, 26 October 2012