Team:UCSF/Protocols

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<regulartext>  
<regulartext>  
<ul>
<ul>
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<li>Primer Design</li>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Creating%20Primers%20on%20ApE.doc">Primer Design for Gibson and Seamless Cloning</a> <br></li>
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<li>Phusion PCR Protocol</li>
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<li>Gibson Assembly Protocol</li>
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<li>Seamless Cloning Protocol</li>
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<li>Colony PCR Protocol</li>
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<li><i>E. coli</i> Transformation</li>
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</ul> <br>
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<h3red>Violacein Production Protocols</h3red> <p><br>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/PCR%20Phusion.pdf">PCR with Phusion Polymerase</a> <br></li>
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<h3red>Auxotroph System Protocols</h3red> <p>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Gibson%20Assembly.pdf">Gibson Assembly</a> <br></li>
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<ul>
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<li>Growing in M9</li>
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<li>Growing Monocultures</li>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/geneart_seamless_cloning_and_assembly_man-1.pdf">Seamless Cloning Protocol</a> <br></li>
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<li>Co-culture Experiments</li>
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<br>
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<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Ecoli%20Transformation.pdf"><i>E. coli</i> Transformation</a> <br></li>
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</ul> <br>
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<h3red>Toxin/Antitoxin System Protocols</h3red><br>
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<regulartext1> The above protocols were used in the development of each part of our project. </regulartext1> <p>
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Latest revision as of 21:31, 3 October 2012

General Cloning Protocols


The above protocols were used in the development of each part of our project.

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