Team:Columbia-Cooper-NYC/Main

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<div style="float: left;">[https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main page] [https://2012.igem.org/wiki/index.php?title=Talk:Team:Columbia-Cooper-NYC&action=edit discussion]  [https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main&action=edit edit] [https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main&action=history history]</div>
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[https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main page]            
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[https://2012.igem.org/wiki/index.php?title=Team:Columbia-Cooper-NYC/Main&action=edit edit]  
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">
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The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we intend to install a light sensitive mechanism which will enable us to etch copper in a desired pattern, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light will be focused on it in a desired pattern. The light sensitive mechanism in ferrooxidans would activate and self-destruct in the pathway of the light. In the end, the circuit board will be "etched" by the bacteria everywhere but the illuminated spots, leaving the desired pattern behind on the circuit board. </p>
+
The Columbia-Cooper iGEM team is working with Acidithiobacillus ferrooxidans to create a light-controlled printed circuit board manufacturing process. This bacteria’s metabolism relies on its ability to oxidize iron; the iron can then be used to oxidize, and in turn solubilize, copper. By genetically altering the bacteria, we will install a light sensitive mechanism which will enable controlled copper etching, leaving a finished circuit board. Once a blank printed circuit board is placed in a thin layer of solid media, the bacteria will be applied onto the surface of the media and light of the appropriate wavelength will be focused on it in a desired pattern. The light sensitive mechanism in A. ferrooxidans will activate a self-destruct mechanism preventing copper etching in these locations. In the end, the circuit board will be "etched" by the bacteria everywhere that is not illuminated, leaving a desired pattern on the circuit board under the cells in the path of the light. Click <a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Overview">here</a> for details. </p>
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<div id="lefttop" style="background:#B87333; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">iGEM</div>
<div id="lefttop" style="background:#B87333; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">iGEM</div>
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<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
<div id="leftcontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> The <a style="color:#6bbe00 !important" href="https://igem.org/Main_Page">International Genetically Engineered Machine competition (iGEM)</a> is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts at the beginning of the summer from the <a style="color:#6bbe00 !important" href="http://partsregistry.org/Main_Page">Registry of Standard Biological Parts</a>. Working at their own schools over the summer, they use these parts and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method. In 2011 iGEM expanded to include a High School Division and an Entrepreneurship Division is 2012.</p>
+
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> The <a style="color:#6bbe00 !important" href="https://igem.org/Main_Page">International Genetically Engineered Machine competition (iGEM)</a> is the premiere undergraduate Synthetic Biology competition. Student teams are given a kit of biological parts from the <a style="color:#6bbe00 !important" href="http://partsregistry.org/Main_Page">Registry of Standard Biological Parts</a>. Working at their own schools over the summer, they use these and new parts of their own design to build biological systems and operate them in living cells. This project design and competition format is an exceptionally motivating and effective teaching method.</p>
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<div id="cbox" style="float:left; height:350px;  width:316px; margin-left:5px;" class="tophome">
<div id="ctop" style="background:#238E23;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Genetics</div>
<div id="ctop" style="background:#238E23;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Genetics</div>
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<div id="ccontent" style="background:-webkit-gradient(linear, left top, left bottom, from(#f2f2f2), to(#f8f8f8));  
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:350px;">
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">About our genetics work.</a>.
+
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">Our Genetics Team was responsible for designing and implementing a series of genes which would allow us to exert light based spatial control of apoptosis in E.coli, and eventually transition into A. ferrooxidans. This design includes the expression of a light sensor, which, when exposed to specific wavelengths of light, triggers transcription of a lysis cassette.  A few of the parts which we experimented with are <a style="color:#6bbe00 !important" href="http://partsregistry.org/Part:BBa_K952000">BBa_K952000</a>, <a style="color:#6bbe00 !important" href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K592016">BBa_K592016</a>, and <a style="color:#6bbe00 !important" href="http://partsregistry.org/Part:BBa_K124017">BBa_K124017</a>.
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<div id="rtop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Copper Etching</div>
<div id="rtop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Copper Etching</div>
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:350px;">
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">About our copper work.</a>.
+
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:77%;"> Our project is to revamp a chemical and mechanical manufacturing process, so in addition to working with DNA, we also worked on macro-scale batches of copper foil, the bacteria A. ferrooxidans. Using our liquid media, we were able to grow ferrooxidans and simultaneously etch copper foil much faster than the basal rate (i.e. copper in the liquid media without ferrooxidans). Since our PCB manufacturing process requires the ferrooxidans to "stay put", we have been developing a solid agar-based media, on which the ferrooxidans grow, and below which the copper is etched, again faster than the basal rate.  Click <a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Columbia_notebook_1">here</a> for details</a>.
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<div id="lefttop" style="background:#238E23; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">Biobrick</div>
<div id="lefttop" style="background:#238E23; background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png');" class="secheader">Biobrick</div>
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:250px;">
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> This is how you link things <a style="color:#6bbe00 !important" href="https://2010.igem.org/Team:Cambridge/Bioluminescence">link</a> blahahahahjhmvgmh.</p>
+
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> Our team submitted multiple composite parts, which were built from preexisting Biobricks and used in our project. We also submitted a novel red light sensing protein domain, FphA753s, which was constructed to conform to the RFC25 – Freiburg fusion standard, and is intended for future use to allow light based control of transcription. Click
 +
 +
<a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Parts_Submitted">here</a> for a full list of our submitted parts.</p>
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<div id="cbox" style="float:left; height:250px;  width:316px; margin-left:5px;" class="tophome">
<div id="ctop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Outreach</div>
<div id="ctop" style="background:#B87333;background-image:url('https://static.igem.org/mediawiki/2010/a/ad/Cam-Gradient-Overlay.png')" class="secheader">Outreach</div>
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background: -moz-linear-gradient(top, #f2f2f2, #f8f8f8); color:#333333; height:250px;">
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<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;">About our genetics work.</a>.
+
<p style="line-height:140%; padding-left:10px; padding-top:10px; padding-right:10px;text-align:justify; font-size:90%;"> Our team organized a booth at the 2012 Maker Faire in New York City to share the work we've been doing with the public. We also made 3 interactive games to teach children about recombinant DNA and the various forms of bacterial transformation. Click <a style="color:#6bbe00 !important" href="https://2012.igem.org/Team:Columbia-Cooper-NYC/Outreach">here</a> for more info.</a>
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Team:Cambridge

From 2010.igem.org


  • Columbia & Cooper 2012 Team

  • Columbia University

  • The Cooper Union for the Advancement of Science and Art

  • Genetics

  • Copper Etching

  • The Effect of A. ferrooxidans

  • Maker Faire (Copyright © Andrew Cribb)

Retrieved from "http://2012.igem.org/Team:Columbia-Cooper-NYC/Main"