Team:UANL Mty-Mexico/Notebook

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This is a template page. READ THESE INSTRUCTIONS.
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<p><br><h3>Cloning Strategy</h3><br></p>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<p><b>Fusion proteins</b></p>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<p>We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.</p>
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!align="center"|[[Team:UANL_Mty-Mexico|Home]]
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!align="center"|[[Team:UANL_Mty-Mexico/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=UANL_Mty-Mexico Official Team Profile]
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!align="center"|[[Team:UANL_Mty-Mexico/Project|Project]]
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!align="center"|[[Team:UANL_Mty-Mexico/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UANL_Mty-Mexico/Modeling|Modeling]]
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!align="center"|[[Team:UANL_Mty-Mexico/Notebook|Notebook]]
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!align="center"|[[Team:UANL_Mty-Mexico/Safety|Safety]]
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!align="center"|[[Team:UANL_Mty-Mexico/Attributions|Attributions]]
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<p><br></p>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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<caption align="bottom"><b>Figure 1.</b> Cloning strategy for contructions of fusion proteins.</caption>
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<tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr>
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For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab.
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<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li><b>>>Cloning Strategy</b></a></li>
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<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li>Wetlab</a></li>
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<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li>
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Latest revision as of 04:06, 27 September 2012

iGEM UANL 2012


Cloning Strategy


Fusion proteins


We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.




Figure 1. Cloning strategy for contructions of fusion proteins.


For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab.



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