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Team:UANL Mty-Mexico/Notebook
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| - | <p><br><h3> | + | <p><br><h3>Cloning Strategy</h3><br></p> |
<p><b>Fusion proteins</b></p> | <p><b>Fusion proteins</b></p> | ||
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| - | <p>We used a cloning strategy similar to the Standard Assembly 21 based on the compatible restriction sites BglII and BamHI. | + | <p>We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.</p> |
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| + | <table class="image" align="center" > | ||
| + | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/a/a5/Q_Sites.png/800px-Q_Sites.png" style="width:600px;"></td></tr> | ||
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<p><br></p> | <p><br></p> | ||
<table class="image" align="center" > | <table class="image" align="center" > | ||
<caption align="bottom"><b>Figure 1.</b> Cloning strategy for contructions of fusion proteins.</caption> | <caption align="bottom"><b>Figure 1.</b> Cloning strategy for contructions of fusion proteins.</caption> | ||
| - | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/ | + | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr> |
</table> | </table> | ||
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| + | <p><br></p> | ||
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| + | For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab. | ||
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| - | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li><b>>> | + | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook"><li><b>>>Cloning Strategy</b></a></li> |
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li>Wetlab</a></li> | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/wetlab"><li>Wetlab</a></li> | ||
<a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li> | <a href="https://2012.igem.org/Team:UANL_Mty-Mexico/Notebook/protocols"><li>Protocols</a></li> | ||
Latest revision as of 04:06, 27 September 2012
Cloning Strategy
Fusion proteins
We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.
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