Team:UC Chile/Cyanolux/Biobricks

From 2012.igem.org

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{{UC_Chile4}}
{{UC_Chile4}}
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<h1>Constructs detail</h1>
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<h1>Plasmid backbones</h1>
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<h2>BBa_K743006</h2>
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<h2>pSB1C3_IntK (BBa_K743006)</h2>
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Neutral recombination plasmid backbone. Kanamycin resistance as a selectable marker for integration. EcoRI restriction site in RS2. Plasmid backbone designed for Gibson Assembly.
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<html><center><img src="https://static.igem.org/mediawiki/2012/6/6f/C4plasmidK743006.jpg" align="middle" width="800"></center></html>
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[[File:UC Chile-IntKplasmidResult.jpg | 400px ]]
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<h2>BBa_K743004 or BBa_K743005</h2>
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<br />
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<html><center><img src="https://static.igem.org/mediawiki/2012/a/a2/UC_Chile-BBa_K743004_OR_BBa_K743005.png" align="middle" width="800"></center></html>
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<h2>pSB1C3_IntS (BBa_K743010)</h2>
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Suceptibility generating plasmid backbone. Spectinomycin resistance as a selectable marker for integration. mRFP1 under constitutive promoter in E.coli for negative selection of standard assembly.
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<br>
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[[File:UC Chile-IntSplasmidResult.jpg | 600px ]]
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Integrative plasmid designed for the expression of RFP under synechocystis psbAB or psaA2 promoter also with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.
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<br />
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<h1>Biobricks and Constructs</h1>
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<br />
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<h2>BBa_K743009 or BBa_K743016</h2>
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<h2>BBa_K743000</h2>
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<html><center><img src="https://static.igem.org/mediawiki/2012/1/1e/UC_Chile-BBa_K743009_or_BBa_K743016.png" align="middle" width="800"></center></html>
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<br />
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First recombination site of IntK plasmid backbone.
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[[File:UC Chile-BBa_K743000.jpg | 230px ]]
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<h2>BBa_K743010</h2>
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<br />
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<html><center><img src="https://static.igem.org/mediawiki/2012/b/b6/UC_Chile-BBa_K743010.png" align="middle" width="800"></center></html>
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<h2>BBa_K743001</h2>
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<br />
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Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly.
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<h2>LuxCDEG_in_IntC</h2>
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[[File:UC Chile-BBa_K743001.jpg | 250px ]]
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<html><center><img src="https://static.igem.org/mediawiki/2012/a/ae/UC_Chile-LuxCDEG_in_IntC.png" align="middle" width="800"></center></html>
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<br />
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<h2>Failed_strategy</h2>
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<h2>BBa_K743002</h2>
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<html><center><img src="https://static.igem.org/mediawiki/2012/a/a7/UC_Chile-Failed_strategy.png" align="middle" width="800"></center></html>
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<br />
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<i>Synechocystis</i> PCC. 6803 Citocrome C promoter.
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[[File:UC Chile-BBa_K743002.jpg | 250px ]]
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<h2>PSB1C3_INTK</h2>
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<br />
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<html><center><img src="https://static.igem.org/mediawiki/2012/8/85/UC_Chile-PSB1C3_INTK.jpg" align="center" middle="800"></center></html>
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<h2>BBa_K743009</h2>
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<br />
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pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsaAB promoter.
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<h2>PSb1C3_INTS</h2>
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[[File:UC Chile-BBa_K743009.jpg | 500px ]]
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<html><center><img src="https://static.igem.org/mediawiki/2012/6/69/UC_Chile-PS81C3_INTS.jpg" align="center" middle="800"></center></html>
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<br />
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== Brief description of characterized biobricks utilized ==
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== Biobricks ==
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<h2>BBa_K743016</h2>
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<br />
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pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsbA2 promoter.
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[[File:UC Chile-BBa_K743016.jpg | 500px ]]
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<br />
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<h2>BBa_K743014</h2>
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<br />
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pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminescent under <i>Synechocystis</i> Transaldolase promoter.
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[[File:UC Chile-IntKplasmidLuxABxl.jpg | 600px ]]
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<br />
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<h2>BBa_K743015</h2>
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<br />
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pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under <i>Synechocystis</i> Transaldolase promoter.
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[[File:UC Chile-IntKfullplasmidResult.jpg | 600px ]]
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<br />
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<h2>BBa_K743018</h2>
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<br />
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pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under <i>Synechocystis</i> Transaldolase promoter.
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[[File:UC Chile-BBa_K743018sfGFPtag.jpg | 600px ]]

Latest revision as of 09:55, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012