Team:ETH Zurich/Cph8/Results

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==Cloning of Cph8==
==Cloning of Cph8==
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We successfully cloned the PCB generator as well as the Cph8 coding region ([http://partsregistry.org/Part:BBa_K909001 K909001]).
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We successfully cloned and sequenced the PCB generator ([http://partsregistry.org/Part:BBa_K909001 K909001]). Furthermore we achieved to isolate the Cph8 coding region from pJT122 by PCR and removed an illegal PstI-Restrictionsite using Site-directed-mutagenesis ([http://partsregistry.org/Part:BBa_K909002 K909002]).
==Plans==
==Plans==
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As a next step we will test our constructs under different light conditions. After the characterization of this part we want to integrate Cph8 in our Decoder construct and test it under different light conditions.   
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As the next steps we will finish the construction of the [https://2012.igem.org/File:Cph8_1.png Reporter and Sensing Plasmids] and will test our constructs under different light conditions. After the characterization of this part we want to integrate the Cph8-system in our Decoder and test it under different light sources.   
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{{:Team:ETH_Zurich/Templates/Footer}}

Latest revision as of 00:30, 27 September 2012

Eth ecolipseeth logo.png
Eth igem logo.png

Cloning of Cph8

We successfully cloned and sequenced the PCB generator ([http://partsregistry.org/Part:BBa_K909001 K909001]). Furthermore we achieved to isolate the Cph8 coding region from pJT122 by PCR and removed an illegal PstI-Restrictionsite using Site-directed-mutagenesis ([http://partsregistry.org/Part:BBa_K909002 K909002]).


Plans

As the next steps we will finish the construction of the Reporter and Sensing Plasmids and will test our constructs under different light conditions. After the characterization of this part we want to integrate the Cph8-system in our Decoder and test it under different light sources.


References

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