Team:UANL Mty-Mexico/Notebook
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- | <p><br><h3> | + | <p><br><h3>Cloning Strategy</h3><br></p> |
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+ | <p><b>Fusion proteins</b></p> | ||
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+ | <p>We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.</p> | ||
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+ | <table class="image" align="center" > | ||
+ | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/a/a5/Q_Sites.png/800px-Q_Sites.png" style="width:600px;"></td></tr> | ||
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+ | <p><br></p> | ||
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+ | <table class="image" align="center" > | ||
+ | <caption align="bottom"><b>Figure 1.</b> Cloning strategy for contructions of fusion proteins.</caption> | ||
+ | <tr><td><img src="https://static.igem.org/mediawiki/2012/thumb/5/5f/Assembly_21-2.png/569px-Assembly_21-2.png" style="width:450px;"></td></tr> | ||
+ | </table> | ||
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+ | <p><br></p> | ||
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+ | For the rest of the constructs Standard Assembly 10 was used. Check our results on the "wet lab" tab. | ||
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+ | <p><br></p> | ||
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Latest revision as of 04:06, 27 September 2012
Cloning Strategy
Fusion proteins
We used a cloning strategy similar to the Standard Assembly 21, based on the compatible restriction sites BglII and BamHI. Briefly, a scar is obtained which translation results in the benefical amino acids glicine and serine (Figure 1). In contrast with the Standar Assembly 21, the XhoI site was replaced with a SpeI site, which allows us to use the Standard Assembly 10 backbones.
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