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Team:SDU-Denmark/labwork/Protocols/gel
From 2012.igem.org
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<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/revtrans">Reverse Transcriptase</a></td> | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/revtrans">Reverse Transcriptase</a></td> | ||
| - | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen"> | + | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen">Mutagenesis</a></td> |
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td> | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td> | ||
| - | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
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<h2>Construct a gel - Medium size</h2> | <h2>Construct a gel - Medium size</h2> | ||
</br> | </br> | ||
| - | < | + | <p> |
| - | 2 | + | 1) Weigh 0.8g agarose </br></br> |
| - | 3 | + | 2) Mix with 100ml TBEx1 solution </br></br> |
| - | 4 | + | 3) Heat in a microwave until all the agarose is dissolved and the solution is clear.</br></br> |
| - | 5 | + | 4) Cool it down a bit before adding Ethidium Bromide (warning: toxic)</br></br> |
| - | 6 | + | 5) Pour the solution into a gel casting mold and use a comb of desired size.</br></br> |
| + | 6) Run at 100V for 40 minutes. | ||
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Latest revision as of 21:15, 26 September 2012
| mRNA Isolation | PCR | Miniprep | Check Digest |
| 3A-Assembly | Colony-PCR | Transformation | Gel-electrophoresis |
| Reverse Transcriptase | Mutagenesis | PCR-,gel clean-up |
Gel Electrophoresis
Construct a gel - Medium size
1) Weigh 0.8g agarose 2) Mix with 100ml TBEx1 solution 3) Heat in a microwave until all the agarose is dissolved and the solution is clear. 4) Cool it down a bit before adding Ethidium Bromide (warning: toxic) 5) Pour the solution into a gel casting mold and use a comb of desired size. 6) Run at 100V for 40 minutes.
