Team:Shenzhen/Biobricks

From 2012.igem.org

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{{:http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2012&group=Shenzhen}}
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<div class="context">
<div class="context">
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<h5>Context 1</h5>
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<h5>Favorite Parts</h5>
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<li> </li><li> </li><li> </li><li> </li><li> </li>
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<ul><li>Best Parts</li></ul>
 +
<ul><p>BBa_K809001, mitochondrial coded GFP</p></ul>
 +
<ul><p>BBa_K809002, mitochondrial replication origin ori7</p></ul>
 +
<ul><p>BBa_K809003, mitochondrial upstream elements (promoter+RBS), from cox1 gene</p></ul>
 +
<ul><li>Best Parts, Engineered</li></ul>
 +
<ul><p>BBa_K809101, mtGFP expression unit</p></ul>
 +
<ul><li>Best Improved Parts</li></ul>
 +
<ul><p>BBa_K809605, lamda-Holin in BBa format, engineered from BBa_K112306 in BBb format</p></ul>
 +
<ul><p>BBa_K809601, GAL promoter without start codon, engineered from BBa_J63006 </p></ul>
 +
 
 +
 
 +
<ul><div class="figurep"><ul><p>Table 1. Favorite parts in project YAO #1.0.</p></ul>
 +
<table><tr><th>-?-</th><th>Name</th><th>Type</th><th>Description</th><th>Designer</th><th>Length</th><th>
 +
</th></tr><tr><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_green">W</td><td>BBa_K809001</td><td>Reporter</td><td>Mitochondrial GFP</td><td width="100px">Qiwu XU</td><td align="right">717</td><td class="blank_col"></td>
 +
</tr><tr><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_green">W</td><td>BBa_K809002</td><td>Other</td><td>ori7 region of mtDNA</td><td width="100px">Qiwu XU</td><td align="right">378</td><td class="blank_col"></td>
 +
</tr><tr><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_green">W</td><td>BBa_K809003</td><td>Regulatory</td><td>Up elements of cox1 gene</td><td width="100px">Qiwu XU</td><td align="right">497</td><td class="blank_col"></td>
 +
</tr><tr><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_green">W</td><td>BBa_K809101</td><td>Composite</td><td>Mitochondrial shuttle plasmid carried mtGFP</td><td width="100px">Senwei TANG, Qiwu XU</td><td align="right">1606</td><td class="blank_col"></td>
 +
</tr><tr><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_green">W</td><td>BBa_K809601</td><td>Regulatory</td><td>GAL promoter (kozak) without start codon ATG</td><td width="100px">Jianhui Gong</td><td align="right">549</td><td class="blank_col"></td>
 +
</tr><tr><td class="status_cell cell_white">&nbsp;</td><td class="status_cell cell_green">W</td><td>BBa_K809605</td><td>Coding</td><td>Lambda Holin in BBa format</td><td width="100px">Jianhui Gong</td><td align="right">318</td><td class="blank_col"></td></tr></table></div></ul>
</div>
</div>
<div class="context">
<div class="context">
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<h5>Context 2</h5>
+
<h5>All Parts</h5>
-
<li>  </li>
+
 
-
<li>  </li>
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<ul><p>We have designed and constructed dozens of BioBricks for the five sub-projects. Some of them have been submitted to PartsRegistry, and some of them have been proved workable. </p></ul>
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<li>  </li>
+
 
-
<li>  </li>
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<ul><div class="figurep"><ul><p>Table 2. All designed parts in project YAO #1.0.</p></ul>
-
<li>  </li>
+
<TABLE><TR><TH>-?-<TH>Name</TH><TH>Type</TH><TH>Description</TH><TH>Designer</TH><TH>Length</TH><TH></TH>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809004</TD><TD>Terminator</TD><TD>Terminator of Yeast mitochondrial gene COB</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>50</TD><TD class ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809005</TD><TD>Terminator</TD><TD>Terminator of Yeast mitochondrial gene COX3</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>49</TD><TD class ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809006</TD><TD>Terminator</TD><TD>Terminator of Yeast mitochondrial gene Q0255</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>65</TD><TD class ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809007</TD><TD>Regulatory</TD><TD>Promoter of Yeast mitochondrial gene COX1/Oli1/15s rRNA</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>11</TD><TD class ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809008</TD><TD>RBS</TD><TD>RBS sequence of yeast mitochondrial gene AI1</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>54</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809009</TD><TD>RBS</TD><TD>RBS sequence of yeast mitochondrial gene OLI1</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>114</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD>BBa_K809010</TD><TD>DNA</TD><TD>Upstream recombination site of cox3</TD><TD width='100px'>Kang KANG</TD><TD ALIGN='right'>&nbsp;</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD>BBa_K809011</TD><TD>DNA</TD><TD>Dowstream recombination site of cox3</TD><TD width='100px'>Kang KANG</TD><TD ALIGN='right'>&nbsp;</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_green'>W</TD><TD>BBa_K809102</TD><TD>Composite</TD><TD>Recombination sequence carried with mtGFP</TD><TD width='100px'>Kang KANG</TD><TD ALIGN='right'>&nbsp;</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809103</TD><TD>Composite</TD><TD>Original termination efficiency test BioBricks for Tcob</TD><TD width='100px'>Kang KANG</TD><TD ALIGN='right'>1278</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809104</TD><TD>Composite</TD><TD>Original termination efficiency test BioBricks for Tcox3</TD><TD width='100px'>Kang KANG</TD><TD ALIGN='right'>1277</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809105</TD><TD>Composite</TD><TD>Original termination efficiency test BioBricks for Tq0255</TD><TD width='100px'>Kang KANG</TD><TD ALIGN='right'>1293</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809106</TD><TD>Coding</TD><TD>Efficiency test of COX3 Terminator</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>911</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809107</TD><TD>Coding</TD><TD>Efficiency test of  COB Terminator</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>912</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809108</TD><TD>Coding</TD><TD>Efficiency test of  Q0255 Terminator</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>911</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809201</TD><TD>Protein_Domain</TD><TD>signal peptide of ZIM17</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>141</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809202</TD><TD>Protein_Domain</TD><TD>signal peptide of FER</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>96</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809203</TD><TD>Protein_Domain</TD><TD>signal peptide of TIM21</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>96</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809204</TD><TD>Protein_Domain</TD><TD>signal peptide of Tom40</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>147</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809205</TD><TD>Protein_Domain</TD><TD>signal peptide of Tom70</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>90</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809206</TD><TD>Protein_Domain</TD><TD>signal peptide of Tom22</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>130</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809207</TD><TD>Protein_Domain</TD><TD>signal peptide of Tom20</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>120</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809208</TD><TD>Coding</TD><TD>Toc34</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1467</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809209</TD><TD>Coding</TD><TD>Toc75</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>2520</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809301</TD><TD>Reporter</TD><TD>RFP + signal peptide of ZIM17 </TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>853</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809302</TD><TD>Reporter</TD><TD>RFP + signal peptide of FER</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>808</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809303</TD><TD>Reporter</TD><TD>RFP + signal peptide of TIM21</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>808</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809304</TD><TD>Reporter</TD><TD>RFP + signal peptide of Tom40</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>859</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809305</TD><TD>Reporter</TD><TD>RFP + signal peptide of Tom70</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>802</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809306</TD><TD>Reporter</TD><TD>RFP + signal peptide of Tom22</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>842</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809308</TD><TD>Coding</TD><TD>GAL promoter + RFP_SPzim17 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1642</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809309</TD><TD>Coding</TD><TD>GAL promoter + RFP_SPfer + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1597</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809310</TD><TD>Coding</TD><TD>GAL promoter + RFP_SPtim21 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1642</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809311</TD><TD>Coding</TD><TD>GAL promoter + RFP_SPtom70 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1591</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809312</TD><TD>Coding</TD><TD>GAL promoter + RFP_SPtom22 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1633</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809313</TD><TD>Reporter</TD><TD>YFP + signal peptide of ZIM17</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>179</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809314</TD><TD>Reporter</TD><TD>YFP + signal peptide of  FER</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>134</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809315</TD><TD>Reporter</TD><TD>YFP + signal peptide of Tim21</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>134</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809316</TD><TD>Reporter</TD><TD>YFP + signal peptide of Tom40</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>185</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809317</TD><TD>Reporter</TD><TD>YFP + signal peptide of Tom70</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>128</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809318</TD><TD>Reporter</TD><TD>YFP + signal peptide of Tom22</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>158</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809319</TD><TD>Coding</TD><TD>GAL promoter + YFP_SPzim17 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>967</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809320</TD><TD>Coding</TD><TD>GAL promoter + YFP_SPfer + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>922</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809321</TD><TD>Coding</TD><TD>GAL promoter + YFP_SPtim21 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>922</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809322</TD><TD>Coding</TD><TD>GAL promoter + YFP_SPtom70 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>916</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809323</TD><TD>Coding</TD><TD>GAL promoter + YFP_SPtom22 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>946</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809324</TD><TD>Coding</TD><TD>signal peptide of Tom70 + Toc34</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1563</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809325</TD><TD>Coding</TD><TD>YFP + signal peptide of Tom70 + Toc34</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>1599</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809326</TD><TD>Coding</TD><TD>signal peptide of Tom40 + Toc75</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>2673</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809327</TD><TD>Coding</TD><TD>YFP + signal peptide of Tom40 + Toc75</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>2711</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809328</TD><TD>Coding</TD><TD>GAL promoter + Toc34_SPtom70 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>2351</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809329</TD><TD>Coding</TD><TD>GAL promoter + Toc75_SPtom40 + ADH1 terminator</TD><TD width='100px'>Luohao Xu</TD><TD ALIGN='right'>3463</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809401</TD><TD>Coding</TD><TD>REX coding sequence</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>777</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809402</TD><TD>Regulatory</TD><TD>Promoter+ROP in mitochondria</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>43</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809403</TD><TD>Regulatory</TD><TD>ROP+Promoter in mitochondria</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>43</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809406</TD><TD>Coding</TD><TD>P+ROP+OLI1 RBS+mtGFP+COX3 terminator</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>943</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809501</TD><TD>Coding</TD><TD>REX+SPzim17</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>924</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809502</TD><TD>Coding</TD><TD>GAL promoter + SP_REX + ADH1 terminator</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>1713</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809504</TD><TD>Coding</TD><TD>ROP+P+OLI1 RBS+mtGFP+COX3 terminator</TD><TD width='100px'>Xiaopeng Xu</TD><TD ALIGN='right'>943</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809602</TD><TD>Coding</TD><TD>T7 RNAP</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>2652</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809603</TD><TD>Coding</TD><TD>Bovine Pancreatic DNase I (in yeast mt codon table)</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3023</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809604</TD><TD>Coding</TD><TD>Bovine Pancreatic DNase I </TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3023</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809606</TD><TD>Regulatory</TD><TD>DLD3 promoter</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>1939</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809607</TD><TD>Coding</TD><TD>human bax protein</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>6942</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809701</TD><TD>Coding</TD><TD>SP of ZIM17 + T7 RNAP</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>2799</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809702</TD><TD>Coding</TD><TD>SP of ZIM17 + Bovine Pancreatic DNase I </TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3172</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809703</TD><TD>Coding</TD><TD>SP of ZIM17 + Holin (lambda phage)</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>465</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809704</TD><TD>Coding</TD><TD>Lambda Holin + hsl1 degradation tag</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>944</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809711</TD><TD>Coding</TD><TD>GAL promoter + kozak + ZIM17+ T7 RNAP + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3587</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809712</TD><TD>Coding</TD><TD>GAL promoter + kozak + T7 RNAP + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3440</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809713</TD><TD>Coding</TD><TD>T7 promoter + yeast mt RBS(AI1)+ GFP + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>1042</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809714</TD><TD>Coding</TD><TD>T7 promoter + yeast mt RBS(AI1) + mtGFP + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>1039</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;&nbsp;</TD><TD>BBa_K809715</TD><TD>Coding</TD><TD>T7 promoter + yeast mt RBS(AI1)+ Bovine Pancreatic DNase I (mt) + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3347</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809716</TD><TD>Coding</TD><TD>GAL promoter + DNase + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3813</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809717</TD><TD>Coding</TD><TD>GAL promoter + ZIM17 + DNase(mt) + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3960</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809718</TD><TD>Coding</TD><TD>GAL promoter + ZIM17 + hoiln(lambda phage) + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>1253</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809719</TD><TD>Coding</TD><TD>DLD3 promoter + holin(lambda phage) + hsl1 tag + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>3148</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809720</TD><TD>Coding</TD><TD>DLD3 promoter + kozak + yeast GFP+ ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>2924</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809721</TD><TD>Coding</TD><TD>DLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>2522</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809722</TD><TD>Coding</TD><TD>DLD3 promoter + kozak + yeast GFP + hsl1 tag  +ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>2229</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809723</TD><TD>Coding</TD><TD>GAL promoter + kozak + hoiln(lambda phage)+ ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>2522</TD><TD class  ='blank_col'></TD></tr>
 +
<TR><TD class='status_cell cell_white'>&nbsp;</TD><TD class='status_cell cell_white'>&nbsp;</TD><TD>BBa_K809724</TD><TD>Coding</TD><TD>GAL promoter + kozak + Bax(human) + ADH1 terminator</TD><TD width='100px'>Jianhui Gong</TD><TD ALIGN='right'>9148</TD><TD class  ='blank_col'></TD></tr>
 +
</TABLE></div></ul>
</div>
</div>
</div>
</div>
{{:Team:Shenzhen/Temp/gallery.htm}}
{{:Team:Shenzhen/Temp/gallery.htm}}

Latest revision as of 16:00, 26 September 2012




Yao.BioBricks.jpg
Favorite Parts
  • Best Parts

    BBa_K809001, mitochondrial coded GFP

    BBa_K809002, mitochondrial replication origin ori7

    BBa_K809003, mitochondrial upstream elements (promoter+RBS), from cox1 gene

  • Best Parts, Engineered

    BBa_K809101, mtGFP expression unit

  • Best Improved Parts

    BBa_K809605, lamda-Holin in BBa format, engineered from BBa_K112306 in BBb format

    BBa_K809601, GAL promoter without start codon, engineered from BBa_J63006


      Table 1. Favorite parts in project YAO #1.0.

    -?-NameTypeDescriptionDesignerLength
     WBBa_K809001ReporterMitochondrial GFPQiwu XU717
     WBBa_K809002Otherori7 region of mtDNAQiwu XU378
     WBBa_K809003RegulatoryUp elements of cox1 geneQiwu XU497
     WBBa_K809101CompositeMitochondrial shuttle plasmid carried mtGFPSenwei TANG, Qiwu XU1606
     WBBa_K809601RegulatoryGAL promoter (kozak) without start codon ATGJianhui Gong549
     WBBa_K809605CodingLambda Holin in BBa formatJianhui Gong318
All Parts

    We have designed and constructed dozens of BioBricks for the five sub-projects. Some of them have been submitted to PartsRegistry, and some of them have been proved workable.

      Table 2. All designed parts in project YAO #1.0.

    -?-NameTypeDescriptionDesignerLength
       BBa_K809004TerminatorTerminator of Yeast mitochondrial gene COBXiaopeng Xu50
       BBa_K809005TerminatorTerminator of Yeast mitochondrial gene COX3Xiaopeng Xu49
       BBa_K809006TerminatorTerminator of Yeast mitochondrial gene Q0255Xiaopeng Xu65
       BBa_K809007RegulatoryPromoter of Yeast mitochondrial gene COX1/Oli1/15s rRNAXiaopeng Xu11
       BBa_K809008RBSRBS sequence of yeast mitochondrial gene AI1Xiaopeng Xu54
       BBa_K809009RBSRBS sequence of yeast mitochondrial gene OLI1Xiaopeng Xu114
     WBBa_K809010DNAUpstream recombination site of cox3Kang KANG 
     WBBa_K809011DNADowstream recombination site of cox3Kang KANG 
     WBBa_K809102CompositeRecombination sequence carried with mtGFPKang KANG 
      BBa_K809103CompositeOriginal termination efficiency test BioBricks for TcobKang KANG1278
      BBa_K809104CompositeOriginal termination efficiency test BioBricks for Tcox3Kang KANG1277
      BBa_K809105CompositeOriginal termination efficiency test BioBricks for Tq0255Kang KANG1293
       BBa_K809106CodingEfficiency test of COX3 TerminatorXiaopeng Xu911
       BBa_K809107CodingEfficiency test of COB TerminatorXiaopeng Xu912
       BBa_K809108CodingEfficiency test of Q0255 TerminatorXiaopeng Xu911
       BBa_K809201Protein_Domainsignal peptide of ZIM17Luohao Xu141
       BBa_K809202Protein_Domainsignal peptide of FERLuohao Xu96
       BBa_K809203Protein_Domainsignal peptide of TIM21Luohao Xu96
       BBa_K809204Protein_Domainsignal peptide of Tom40Luohao Xu147
       BBa_K809205Protein_Domainsignal peptide of Tom70Luohao Xu90
       BBa_K809206Protein_Domainsignal peptide of Tom22Luohao Xu130
      BBa_K809207Protein_Domainsignal peptide of Tom20Luohao Xu120
       BBa_K809208CodingToc34Luohao Xu1467
       BBa_K809209CodingToc75Luohao Xu2520
      BBa_K809301ReporterRFP + signal peptide of ZIM17 Luohao Xu853
       BBa_K809302ReporterRFP + signal peptide of FERLuohao Xu808
       BBa_K809303ReporterRFP + signal peptide of TIM21Luohao Xu808
       BBa_K809304ReporterRFP + signal peptide of Tom40Luohao Xu859
       BBa_K809305ReporterRFP + signal peptide of Tom70Luohao Xu802
       BBa_K809306ReporterRFP + signal peptide of Tom22Luohao Xu842
      BBa_K809308CodingGAL promoter + RFP_SPzim17 + ADH1 terminatorLuohao Xu1642
      BBa_K809309CodingGAL promoter + RFP_SPfer + ADH1 terminatorLuohao Xu1597
      BBa_K809310CodingGAL promoter + RFP_SPtim21 + ADH1 terminatorLuohao Xu1642
      BBa_K809311CodingGAL promoter + RFP_SPtom70 + ADH1 terminatorLuohao Xu1591
      BBa_K809312CodingGAL promoter + RFP_SPtom22 + ADH1 terminatorLuohao Xu1633
       BBa_K809313ReporterYFP + signal peptide of ZIM17Luohao Xu179
       BBa_K809314ReporterYFP + signal peptide of FERLuohao Xu134
       BBa_K809315ReporterYFP + signal peptide of Tim21Luohao Xu134
       BBa_K809316ReporterYFP + signal peptide of Tom40Luohao Xu185
       BBa_K809317ReporterYFP + signal peptide of Tom70Luohao Xu128
       BBa_K809318ReporterYFP + signal peptide of Tom22Luohao Xu158
      BBa_K809319CodingGAL promoter + YFP_SPzim17 + ADH1 terminatorLuohao Xu967
      BBa_K809320CodingGAL promoter + YFP_SPfer + ADH1 terminatorLuohao Xu922
      BBa_K809321CodingGAL promoter + YFP_SPtim21 + ADH1 terminatorLuohao Xu922
      BBa_K809322CodingGAL promoter + YFP_SPtom70 + ADH1 terminatorLuohao Xu916
      BBa_K809323CodingGAL promoter + YFP_SPtom22 + ADH1 terminatorLuohao Xu946
       BBa_K809324Codingsignal peptide of Tom70 + Toc34Luohao Xu1563
      BBa_K809325CodingYFP + signal peptide of Tom70 + Toc34Luohao Xu1599
       BBa_K809326Codingsignal peptide of Tom40 + Toc75Luohao Xu2673
      BBa_K809327CodingYFP + signal peptide of Tom40 + Toc75Luohao Xu2711
      BBa_K809328CodingGAL promoter + Toc34_SPtom70 + ADH1 terminatorLuohao Xu2351
      BBa_K809329CodingGAL promoter + Toc75_SPtom40 + ADH1 terminatorLuohao Xu3463
       BBa_K809401CodingREX coding sequenceXiaopeng Xu777
       BBa_K809402RegulatoryPromoter+ROP in mitochondriaXiaopeng Xu43
       BBa_K809403RegulatoryROP+Promoter in mitochondriaXiaopeng Xu43
       BBa_K809406CodingP+ROP+OLI1 RBS+mtGFP+COX3 terminatorXiaopeng Xu943
       BBa_K809501CodingREX+SPzim17Xiaopeng Xu924
       BBa_K809502CodingGAL promoter + SP_REX + ADH1 terminatorXiaopeng Xu1713
       BBa_K809504CodingROP+P+OLI1 RBS+mtGFP+COX3 terminatorXiaopeng Xu943
      BBa_K809602CodingT7 RNAPJianhui Gong2652
      BBa_K809603CodingBovine Pancreatic DNase I (in yeast mt codon table)Jianhui Gong3023
      BBa_K809604CodingBovine Pancreatic DNase I Jianhui Gong3023
       BBa_K809606RegulatoryDLD3 promoterJianhui Gong1939
      BBa_K809607Codinghuman bax proteinJianhui Gong6942
       BBa_K809701CodingSP of ZIM17 + T7 RNAPJianhui Gong2799
      BBa_K809702CodingSP of ZIM17 + Bovine Pancreatic DNase I Jianhui Gong3172
       BBa_K809703CodingSP of ZIM17 + Holin (lambda phage)Jianhui Gong465
       BBa_K809704CodingLambda Holin + hsl1 degradation tagJianhui Gong944
       BBa_K809711CodingGAL promoter + kozak + ZIM17+ T7 RNAP + ADH1 terminatorJianhui Gong3587
       BBa_K809712CodingGAL promoter + kozak + T7 RNAP + ADH1 terminatorJianhui Gong3440
       BBa_K809713CodingT7 promoter + yeast mt RBS(AI1)+ GFP + ADH1 terminatorJianhui Gong1042
      BBa_K809714CodingT7 promoter + yeast mt RBS(AI1) + mtGFP + ADH1 terminatorJianhui Gong1039
       BBa_K809715CodingT7 promoter + yeast mt RBS(AI1)+ Bovine Pancreatic DNase I (mt) + ADH1 terminatorJianhui Gong3347
      BBa_K809716CodingGAL promoter + DNase + ADH1 terminatorJianhui Gong3813
      BBa_K809717CodingGAL promoter + ZIM17 + DNase(mt) + ADH1 terminatorJianhui Gong3960
      BBa_K809718CodingGAL promoter + ZIM17 + hoiln(lambda phage) + ADH1 terminatorJianhui Gong1253
      BBa_K809719CodingDLD3 promoter + holin(lambda phage) + hsl1 tag + ADH1 terminatorJianhui Gong3148
      BBa_K809720CodingDLD3 promoter + kozak + yeast GFP+ ADH1 terminatorJianhui Gong2924
      BBa_K809721CodingDLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminatorJianhui Gong2522
      BBa_K809722CodingDLD3 promoter + kozak + yeast GFP + hsl1 tag +ADH1 terminatorJianhui Gong2229
      BBa_K809723CodingGAL promoter + kozak + hoiln(lambda phage)+ ADH1 terminatorJianhui Gong2522
      BBa_K809724CodingGAL promoter + kozak + Bax(human) + ADH1 terminatorJianhui Gong9148