Team:UC Chile/Cyanolux/Biobricks
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- | <h1> | + | <h1>Plasmid backbones</h1> |
+ | <h2>pSB1C3_IntK (BBa_K743006)</h2> | ||
+ | Neutral recombination plasmid backbone. Kanamycin resistance as a selectable marker for integration. EcoRI restriction site in RS2. Plasmid backbone designed for Gibson Assembly. | ||
- | + | [[File:UC Chile-IntKplasmidResult.jpg | 400px ]] | |
- | + | ||
- | <br> | + | <br /> |
- | + | <h2>pSB1C3_IntS (BBa_K743010)</h2> | |
+ | Suceptibility generating plasmid backbone. Spectinomycin resistance as a selectable marker for integration. mRFP1 under constitutive promoter in E.coli for negative selection of standard assembly. | ||
+ | [[File:UC Chile-IntSplasmidResult.jpg | 600px ]] | ||
+ | <br /> | ||
- | < | + | <h1>Biobricks and Constructs</h1> |
- | < | + | <br /> |
+ | <h2>BBa_K743000</h2> | ||
+ | <br /> | ||
+ | First recombination site of IntK plasmid backbone. | ||
- | + | [[File:UC Chile-BBa_K743000.jpg | 230px ]] | |
- | + | ||
+ | <br /> | ||
- | <h2> | + | <h2>BBa_K743001</h2> |
- | < | + | <br /> |
+ | Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly. | ||
+ | [[File:UC Chile-BBa_K743001.jpg | 250px ]] | ||
- | < | + | <br /> |
- | + | ||
+ | <h2>BBa_K743002</h2> | ||
+ | <br /> | ||
+ | <i>Synechocystis</i> PCC. 6803 Citocrome C promoter. | ||
- | + | [[File:UC Chile-BBa_K743002.jpg | 250px ]] | |
- | + | ||
+ | <br /> | ||
- | <h2> | + | <h2>BBa_K743009</h2> |
- | < | + | <br /> |
+ | pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsaAB promoter. | ||
- | + | [[File:UC Chile-BBa_K743009.jpg | 500px ]] | |
- | + | ||
- | == | + | <br /> |
+ | |||
+ | <h2>BBa_K743016</h2> | ||
+ | <br /> | ||
+ | pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsbA2 promoter. | ||
+ | |||
+ | [[File:UC Chile-BBa_K743016.jpg | 500px ]] | ||
+ | |||
+ | <br /> | ||
+ | |||
+ | <h2>BBa_K743014</h2> | ||
+ | <br /> | ||
+ | pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminescent under <i>Synechocystis</i> Transaldolase promoter. | ||
+ | [[File:UC Chile-IntKplasmidLuxABxl.jpg | 600px ]] | ||
+ | |||
+ | <br /> | ||
+ | |||
+ | <h2>BBa_K743015</h2> | ||
+ | <br /> | ||
+ | pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under <i>Synechocystis</i> Transaldolase promoter. | ||
+ | |||
+ | [[File:UC Chile-IntKfullplasmidResult.jpg | 600px ]] | ||
+ | |||
+ | <br /> | ||
+ | |||
+ | <h2>BBa_K743018</h2> | ||
+ | <br /> | ||
+ | pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under <i>Synechocystis</i> Transaldolase promoter. | ||
+ | |||
+ | [[File:UC Chile-BBa_K743018sfGFPtag.jpg | 600px ]] | ||
+ | |||
+ | |||
+ | <html> | ||
+ | <a href="https://2012.igem.org/Team:UC_Chile/Cyanolux/Notepad"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right"> | ||
+ | </html> | ||
{{UC_Chilefooter}} | {{UC_Chilefooter}} |
Latest revision as of 09:55, 26 October 2012