Team:HKUST-Hong Kong/Glossary

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           <div><p align="center"><font size="20">GLOSSARY</font></p></div>
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           <div><p align="center"><font size="20">Glossary</font></p></div>
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           <p>Signal peptide: transient &lsquo;zip code&rsquo; of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p>
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           <p><strong>Signal peptide</strong> – transient &lsquo;zip code&rsquo; of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p>
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<p>YbdN: Signal peptide of protein YbdN in <em>Bacillus subtilis.</em></p>
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<p><strong>YbdN</strong> – YbdN stated throughout this wiki refers to the first 24 amino acid residues of the protein YbdN in <em>Bacillus subtilis</em>, which fucntions as a signal peptide.</p>
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<p>ybdN: Gene that encodes YbdN.</p>
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<p><strong><i>ybdN</i></strong> – Gene that codes for YbdN.</p>
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<p>YdjM: Signal peptide from protein YdjM in <em>Bacillus subtilis.</em></p>
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<p><strong>YdjM</strong> – Signal peptide from protein YdjM in <em>Bacillus subtilis.</em></p>
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<p>ydjM: Gene that encodes YdjM.</p>
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<p><strong><i>ydjM</i></strong> – Gene that codes for YdjM.</p>
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<p>BMP-2: Bone morphogenetic protein 2.</p>
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<p><strong>BMP2</strong> – Bone morphogenetic protein 2.</p>
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<p>LytC: <em>B.  subtilis </em>inherent cell-wall associated protein mediating cell-wall turnover.</p>
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<p><strong>LytC</strong> – <em>B.  subtilis </em>inherent cell-wall associated hydrolase mediating cell-wall turnover.</p>
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<p>lytC: Gene that encodes LytC.</p>
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<p><strong><i>lytC</i></strong> – Gene that codes for the LytC hydrolase.</p>
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<p>(EAAAK)n type helical linker: Stiff, long helical linker designed to separate fusion  proteins with minimal disturbance to the function of both proteins. </p>
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<p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion  proteins with minimal disturbance to the function of both proteins. </p>
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<p>RPMrel: a nine amino acid,  disulfide-constrained peptide, CPIEDRPMC (RPMrel), that selectively stained  HT29 colon carcinoma cells.</p>
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<p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT-29 colon carcinoma cells.</p>
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<p>FLAG: &nbsp;8 amino acid peptide tag used in attachment to the protein-of-interest to facilitate multiple assays.</p>
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<p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p>
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<p>PxylA: The region containing the sigma A  binding region for the xylose inducible promoter.</p>
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<p><strong><em>PxylA</em></strong> – The region containing the sigma A  binding region for the xylose inducible promoter.</p>
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<p>XylR: The transcriptional regulator for  PxylA promoter.</p>
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<p><strong>XylR</strong> – The transcriptional regulator for  PxylA promoter.</p>
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<p>pTms: A low efficient constitutive promoter  in <em>E. coli</em> and <em>B. subtilis.</em></p>
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<p><strong><em>Ptms</em></strong> – A low efficient constitutive promoter  in <em>E. coli</em> and <em>B. subtilis.</em></p>
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<p>EndoA: an endoribonuclease can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).</p>
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<p><strong>EndoA</strong> – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).</p>
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<p>ydcE (ndoA): Gene that encodes EndoA which cleave  the transcript of threonyl-tRNA synthetase (thrS).<br />
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<p><strong><i>ydcE</i> (<i>ndoA</i>)</strong> – Gene that codes for the EndoA endoribonuclease.<br /></p>
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<p><strong>YdcD</strong> – Antidote that counteracts the function of EndoA.</p>
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<p>YdcD: Antidote that counteracts with EndoA.</p>
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<p><strong><i>ydcD</i> (<i>endB</i>)</strong> – Gene that codes for the YdcD antidote. </p>
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<p>ydcD (endB): Gene that encodes YdcD. </p>
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Latest revision as of 23:18, 26 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

Glossary

Signal peptide – transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.

YbdN – YbdN stated throughout this wiki refers to the first 24 amino acid residues of the protein YbdN in Bacillus subtilis, which fucntions as a signal peptide.

ybdN – Gene that codes for YbdN.

YdjM – Signal peptide from protein YdjM in Bacillus subtilis.

ydjM – Gene that codes for YdjM.

BMP2 – Bone morphogenetic protein 2.

LytC – B. subtilis inherent cell-wall associated hydrolase mediating cell-wall turnover.

lytC – Gene that codes for the LytC hydrolase.

(EAAAK)n type helical linker – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.

RPMrel – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT-29 colon carcinoma cells.

FLAG™ – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.

PxylA – The region containing the sigma A binding region for the xylose inducible promoter.

XylR – The transcriptional regulator for PxylA promoter.

Ptms – A low efficient constitutive promoter in E. coli and B. subtilis.

EndoA – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).

ydcE (ndoA) – Gene that codes for the EndoA endoribonuclease.

YdcD – Antidote that counteracts the function of EndoA.

ydcD (endB) – Gene that codes for the YdcD antidote.

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