"
Team:SDU-Denmark/labwork/Protocols/Checkdigest
From 2012.igem.org
(Difference between revisions)
| (One intermediate revision not shown) | |||
| Line 263: | Line 263: | ||
<tr> | <tr> | ||
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/revtrans">Reverse Transcriptase</a></td> | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/revtrans">Reverse Transcriptase</a></td> | ||
| - | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen"> | + | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/mutagen">Mutagenesis</a></td> |
<td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td> | <td><a href="https://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/pcrgelclean">PCR-,gel clean-up</a></td> | ||
| - | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 21:09, 26 September 2012
| mRNA Isolation | PCR | Miniprep | Check Digest |
| 3A-Assembly | Colony-PCR | Transformation | Gel-electrophoresis |
| Reverse Transcriptase | Mutagenesis | PCR-,gel clean-up |
Check Digest
Digest mix, using Fastdigest enzymes
Digest1) Cast an agarose gel for purification of the cut product 2) Mix the digestion mixture in an eppendorf tube 3) Leave for 5 min. at 37°C (no shaking!) 4) Immidiately load the restriction mixture in the gel 5) Run the gel, cut out and purify bands of correct size. Digestion Mix
4 µL H2O 0,5 µL enzyme A 0,5 µL enzyme B 2 µL Fast Digest green buffer 3 µL dna product
